Six2 Defines and Regulates a Multipotent Self-Renewing Nephron Progenitor Population throughout Mammalian Kidney Development

Akio Kobayashi, M. Todd Valerius, Joshua W. Mugford, Thomas J. Carroll, Michelle Self, Guillermo Oliver, Andrew P. McMahon

Research output: Contribution to journalArticle

515 Citations (Scopus)

Abstract

Nephrons, the basic functional units of the kidney, are generated repetitively during kidney organogenesis from a mesenchymal progenitor population. Which cells within this pool give rise to nephrons and how multiple nephron lineages form during this protracted developmental process are unclear. We demonstrate that the Six2-expressing cap mesenchyme represents a multipotent nephron progenitor population. Six2-expressing cells give rise to all cell types of the main body of the nephron during all stages of nephrogenesis. Pulse labeling of Six2-expressing nephron progenitors at the onset of kidney development suggests that the Six2-expressing population is maintained by self-renewal. Clonal analysis indicates that at least some Six2-expressing cells are multipotent, contributing to multiple domains of the nephron. Furthermore, Six2 functions cell autonomously to maintain a progenitor cell status, as cap mesenchyme cells lacking Six2 activity contribute to ectopic nephron tubules, a mechanism dependent on a Wnt9b inductive signal. Taken together, our observations suggest that Six2 activity cell-autonomously regulates a multipotent nephron progenitor population.

Original languageEnglish (US)
Pages (from-to)169-181
Number of pages13
JournalCell Stem Cell
Volume3
Issue number2
DOIs
StatePublished - Aug 7 2008

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Nephrons
Kidney
Population
Mesoderm
Organogenesis
Stem Cells

Keywords

  • DEVBIO
  • STEMCELL

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Medicine
  • Genetics

Cite this

Six2 Defines and Regulates a Multipotent Self-Renewing Nephron Progenitor Population throughout Mammalian Kidney Development. / Kobayashi, Akio; Valerius, M. Todd; Mugford, Joshua W.; Carroll, Thomas J.; Self, Michelle; Oliver, Guillermo; McMahon, Andrew P.

In: Cell Stem Cell, Vol. 3, No. 2, 07.08.2008, p. 169-181.

Research output: Contribution to journalArticle

Kobayashi, Akio ; Valerius, M. Todd ; Mugford, Joshua W. ; Carroll, Thomas J. ; Self, Michelle ; Oliver, Guillermo ; McMahon, Andrew P. / Six2 Defines and Regulates a Multipotent Self-Renewing Nephron Progenitor Population throughout Mammalian Kidney Development. In: Cell Stem Cell. 2008 ; Vol. 3, No. 2. pp. 169-181.
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