Small trypanosome RNA-binding proteins TbUBP1 and TbUBP2 influence expression of F-box protein mRNAs in bloodstream trypanosomes

Claudia Hartmann, Corinna Benz, Stefanie Brems, Louise Ellis, Van Duc Luu, Mhairi Stewart, Iván D'Orso, Christian Busold, Kurt Fellenberg, Alberto C C Frasch, Mark Carrington, Jörg Hoheisel, Christine E. Clayton

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

In the African trypanosome Trypanosoma brucei nearly all control of gene expression is posttranscriptional; sequences in the 3′-untranslated regions of mRNAs determine the steady-state mRNA levels by regulation of RNA turnover. Here we investigate the roles of two related proteins, TbUBP1 and TbUBP2, containing a single RNA recognition motif, in trypanosome gene expression. TbUBP1 and TbUBP2 are in the cytoplasm and nucleus, comprise ca. 0.1% of the total protein, and are not associated with polysomes or RNA degradation enzymes. Overexpression of TbUBP2 upregulated the levels of several mRNAs potentially involved in cell division, including the CFB1 mRNA, which encodes a protein with a cyclin F-box domain. CFB1 regulation was mediated by the 3′-untranslated region and involved stabilization of the mRNA. Depletion of TbUBP2 and TbUBP1 inhibited growth and downregulated expression of the cyclin F box protein gene CFB2; trans splicing was unaffected. The results of pull-down assays indicated that all tested mRNAs were bound to TbUBP2 or TbUBP1, with some preference for CFB1. We suggest that TbUBP1 and TbUBP2 may be relatively nonspecific RNA-binding proteins and that specific effects of overexpression or depletion could depend on competition between various different proteins for RNA binding.

Original languageEnglish (US)
Pages (from-to)1964-1978
Number of pages15
JournalEukaryotic Cell
Volume6
Issue number11
DOIs
StatePublished - Nov 2007

Fingerprint

F-Box Proteins
F-box proteins
RNA-binding proteins
RNA-Binding Proteins
Trypanosomiasis
blood flow
cyclins
3' untranslated regions
RNA
Messenger RNA
trans-splicing
Trypanosoma brucei
gene expression
polyribosomes
Cyclins
proteins
3' Untranslated Regions
cell division
cytoplasm
Trans-Splicing

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Microbiology

Cite this

Hartmann, C., Benz, C., Brems, S., Ellis, L., Luu, V. D., Stewart, M., ... Clayton, C. E. (2007). Small trypanosome RNA-binding proteins TbUBP1 and TbUBP2 influence expression of F-box protein mRNAs in bloodstream trypanosomes. Eukaryotic Cell, 6(11), 1964-1978. https://doi.org/10.1128/EC.00279-07

Small trypanosome RNA-binding proteins TbUBP1 and TbUBP2 influence expression of F-box protein mRNAs in bloodstream trypanosomes. / Hartmann, Claudia; Benz, Corinna; Brems, Stefanie; Ellis, Louise; Luu, Van Duc; Stewart, Mhairi; D'Orso, Iván; Busold, Christian; Fellenberg, Kurt; Frasch, Alberto C C; Carrington, Mark; Hoheisel, Jörg; Clayton, Christine E.

In: Eukaryotic Cell, Vol. 6, No. 11, 11.2007, p. 1964-1978.

Research output: Contribution to journalArticle

Hartmann, C, Benz, C, Brems, S, Ellis, L, Luu, VD, Stewart, M, D'Orso, I, Busold, C, Fellenberg, K, Frasch, ACC, Carrington, M, Hoheisel, J & Clayton, CE 2007, 'Small trypanosome RNA-binding proteins TbUBP1 and TbUBP2 influence expression of F-box protein mRNAs in bloodstream trypanosomes', Eukaryotic Cell, vol. 6, no. 11, pp. 1964-1978. https://doi.org/10.1128/EC.00279-07
Hartmann, Claudia ; Benz, Corinna ; Brems, Stefanie ; Ellis, Louise ; Luu, Van Duc ; Stewart, Mhairi ; D'Orso, Iván ; Busold, Christian ; Fellenberg, Kurt ; Frasch, Alberto C C ; Carrington, Mark ; Hoheisel, Jörg ; Clayton, Christine E. / Small trypanosome RNA-binding proteins TbUBP1 and TbUBP2 influence expression of F-box protein mRNAs in bloodstream trypanosomes. In: Eukaryotic Cell. 2007 ; Vol. 6, No. 11. pp. 1964-1978.
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