TY - JOUR
T1 - Solution NMR spectroscopy beyond 25 kDa
AU - Kay, Lewis E.
AU - Gardner, Kevin H.
N1 - Copyright:
Copyright 2018 Elsevier B.V., All rights reserved.
PY - 1997/10
Y1 - 1997/10
N2 - Improvements in NMR instrumentation, higher magnetic field strengths, novel NMR experiments and new deuterium-labeling strategies have significantly increased the scope of structural problems that can now be addressed by solution NMR methods. To date, a number of structures of proteins of 30 kDa have been solved using multidimensional 15N,13C,2H NMR techniques, and this molecular weight limit will probably be surpassed in the near future.
AB - Improvements in NMR instrumentation, higher magnetic field strengths, novel NMR experiments and new deuterium-labeling strategies have significantly increased the scope of structural problems that can now be addressed by solution NMR methods. To date, a number of structures of proteins of 30 kDa have been solved using multidimensional 15N,13C,2H NMR techniques, and this molecular weight limit will probably be surpassed in the near future.
UR - http://www.scopus.com/inward/record.url?scp=0030735988&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030735988&partnerID=8YFLogxK
U2 - 10.1016/S0959-440X(97)80084-X
DO - 10.1016/S0959-440X(97)80084-X
M3 - Article
C2 - 9345633
AN - SCOPUS:0030735988
SN - 0959-440X
VL - 7
SP - 722
EP - 731
JO - Current Opinion in Structural Biology
JF - Current Opinion in Structural Biology
IS - 5
ER -