Solution structure of the C-terminal domain from poly(A)-binding protein in trypanosoma cruzi: A vegetal PABC domain

Nadeem Siddiqui, Guennadi Kozlov, Iván D'Orso, Jean François Trempe, Kalle Gehring

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

PABC is a phylogenetically conserved peptide-binding domain primarily found within the C terminus of poly(A)-binding proteins (PABPs). This domain recruits a series of translation factors including poly(A)-interacting proteins (Paip1 and Paip2) and release factor 3 (RF3/GSPT) to the initiation complex on mRNA. Here, we determine the solution structure of the Trypanosoma cruzi PABC domain (TcPABC), a representative of the vegetal class of PABP proteins. TcPABC is similar to human PABC (hPABC) and consists of five α-helices, in contrast to the four helices observed in PABC domains from yeast (yPABC) and hyper plastic disk proteins (hHYD). A mobile N-terminal helix is observed in TcPABC that does not pack against the core of the protein, as found in hPABC. Characteristic to all PABC domains, the last four helices of TcPABC fold into a right-handed super coil. TcPABC demonstrates high-affinity binding to PABP interacting motif-2 (PAM-2) and reveals a peptide-binding surface homologous to that of hPABC. Our results demonstrate the last four helices in TcPABC are sufficient for peptide recognition and we predict a similar binding mode in PABC domains. Furthermore, these results point to the presence of putative PAM-2 site-containing proteins in trypanosomes.

Original languageEnglish (US)
Pages (from-to)1925-1933
Number of pages9
JournalProtein Science
Volume12
Issue number9
DOIs
StatePublished - Sep 1 2003

Fingerprint

Poly(A)-Binding Proteins
Trypanosoma cruzi
Proteins
Peptides
Poly A
Amino Acid Motifs
Trypanosomiasis
Yeast
Plastics
Yeasts
Messenger RNA

Keywords

  • NMR
  • PABC domains
  • Poly(A)-binding proteins
  • Translation factors
  • Trypanosomes

ASJC Scopus subject areas

  • Biochemistry

Cite this

Solution structure of the C-terminal domain from poly(A)-binding protein in trypanosoma cruzi : A vegetal PABC domain. / Siddiqui, Nadeem; Kozlov, Guennadi; D'Orso, Iván; Trempe, Jean François; Gehring, Kalle.

In: Protein Science, Vol. 12, No. 9, 01.09.2003, p. 1925-1933.

Research output: Contribution to journalArticle

Siddiqui, Nadeem ; Kozlov, Guennadi ; D'Orso, Iván ; Trempe, Jean François ; Gehring, Kalle. / Solution structure of the C-terminal domain from poly(A)-binding protein in trypanosoma cruzi : A vegetal PABC domain. In: Protein Science. 2003 ; Vol. 12, No. 9. pp. 1925-1933.
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