Specific and nonspecific inhibition of transcription by DNA, PNA, and phosphorothioate promoter analog duplexes

Susan E. Hamilton, Mridula Iyer, James C. Norton, David R. Corey

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

DNA duplexes analogous to the promoters for SP6 or T7 RNA polymerase inhibit transcription with exquisite selectivity. By contrast, phosphorothioate oligomers inhibit nonselectively, and peptide nucleic acid (PNA) duplexes and PNA:DNA heteroduplexes do not inhibit at all. The absence of recognition of proteins by PNAs may prove to be a substantial advantage for their use as anti-sense agents and nucleic acid probes.

Original languageEnglish (US)
Pages (from-to)2897-2900
Number of pages4
JournalBioorganic and Medicinal Chemistry Letters
Volume6
Issue number23
DOIs
StatePublished - Dec 3 1996

Fingerprint

Peptide Nucleic Acids
Transcription
Nucleic Acid Heteroduplexes
Nucleic Acid Probes
DNA
Oligomers
Proteins
bacteriophage T7 RNA polymerase

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Organic Chemistry
  • Drug Discovery
  • Pharmaceutical Science

Cite this

Specific and nonspecific inhibition of transcription by DNA, PNA, and phosphorothioate promoter analog duplexes. / Hamilton, Susan E.; Iyer, Mridula; Norton, James C.; Corey, David R.

In: Bioorganic and Medicinal Chemistry Letters, Vol. 6, No. 23, 03.12.1996, p. 2897-2900.

Research output: Contribution to journalArticle

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