Specific and nonspecific inhibition of transcription by DNA, PNA, and phosphorothioate promoter analog duplexes

Susan E. Hamilton, Mridula Iyer, James C. Norton, David R. Corey

Research output: Contribution to journalArticle

41 Scopus citations

Abstract

DNA duplexes analogous to the promoters for SP6 or T7 RNA polymerase inhibit transcription with exquisite selectivity. By contrast, phosphorothioate oligomers inhibit nonselectively, and peptide nucleic acid (PNA) duplexes and PNA:DNA heteroduplexes do not inhibit at all. The absence of recognition of proteins by PNAs may prove to be a substantial advantage for their use as anti-sense agents and nucleic acid probes.

Original languageEnglish (US)
Pages (from-to)2897-2900
Number of pages4
JournalBioorganic and Medicinal Chemistry Letters
Volume6
Issue number23
DOIs
StatePublished - Dec 3 1996

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ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Pharmaceutical Science
  • Drug Discovery
  • Clinical Biochemistry
  • Organic Chemistry

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