Specific binding of T lymphocytes to macrophages. I. Kinetics of binding

M. F. Lipscomb, S. Z. Ben Sasson, J. W. Uhr

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Abstract

Peritoneal exudate lymphocytes obtained from immune guinea pigs and cultured for 1 wk on antigen-pulsed autologous macrophages were tested for their ability to bind to fresh antigen-pulsed autologous macrophages or to macrophages pulsed with an irrelevant antigen. Up to 30% of the lymphocytes bound to macrophages bearing the relevant antigen whereas only 2 to 5% remained nonspecifically bound to macrophages after vigorous washing. Specific binding was observed in cultures as early as 1 hr. Analysis of the kinetics of binding suggests that the observed nonspecific binding is not a step in specific binding. The possibility that weaker antigen-independent association between lymphocytes and macrophages precedes specific binding cannot be excluded. No evidence was obtained that serum antibody adsorbed to the macrophage or T cell plays a role in this cell interaction or that the T cell can bind antigen directly. It is suggested that the observed specific binding represents the initial event in stimulation of T lymphocytes by antigen.

Original languageEnglish (US)
Pages (from-to)1748-1754
Number of pages7
JournalJournal of Immunology
Volume118
Issue number5
StatePublished - Dec 1 1977

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ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Lipscomb, M. F., Ben Sasson, S. Z., & Uhr, J. W. (1977). Specific binding of T lymphocytes to macrophages. I. Kinetics of binding. Journal of Immunology, 118(5), 1748-1754.