Specific binding of T lymphocytes to macrophages. III. Spontaneous dissociation of T cells from antigen-pulsed macrophages

S. Z. Ben-Sasson, M. F. Lipscomb, T. F. Tucker, J. W. Uhr

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Peritoneal exudate lymphocytes (PEL) from immune guinea pigs that adhere to antigen-pulsed macrophages (M∅) were cultured for 1 week to yield a population enriched in antigen-specific (selected) T cells. These cells bind specifically within hours to fresh autologous antigen-pulsed M∅. The dissociation of these selected PEL from antigen-pulsed M∅ was studied. No evidence was obtained that factors in the culture medium play a role in dissociation. Lymphocytes that have dissociated from antigen-pulsed M∅ are usually fully capable of rebinding to M∅ freshly pulsed with antigen, suggesting that there is no deficiency in the lymphocytes ability to bind. In contrast, readding antigen to cultures during incubation prevents the predicted dissociation. Moreover, repulsing M∅ cultured without selected PEL restores their capacity to bind fresh selected PEL. These findings indicate that decay of antigen associated with M∅ is the major mechanism underlying the observed dissociation.

Original languageEnglish (US)
Pages (from-to)1902-1906
Number of pages5
JournalJournal of Immunology
Volume120
Issue number6
StatePublished - 1978

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Viral Tumor Antigens
Macrophages
T-Lymphocytes
Antigens
Exudates and Transudates
Lymphocytes
Autoantigens
Culture Media
Guinea Pigs
Population

ASJC Scopus subject areas

  • Immunology

Cite this

Specific binding of T lymphocytes to macrophages. III. Spontaneous dissociation of T cells from antigen-pulsed macrophages. / Ben-Sasson, S. Z.; Lipscomb, M. F.; Tucker, T. F.; Uhr, J. W.

In: Journal of Immunology, Vol. 120, No. 6, 1978, p. 1902-1906.

Research output: Contribution to journalArticle

Ben-Sasson, S. Z. ; Lipscomb, M. F. ; Tucker, T. F. ; Uhr, J. W. / Specific binding of T lymphocytes to macrophages. III. Spontaneous dissociation of T cells from antigen-pulsed macrophages. In: Journal of Immunology. 1978 ; Vol. 120, No. 6. pp. 1902-1906.
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