Specific binding of T lymphocytes to macrophages. III. Spontaneous dissociation of T cells from antigen-pulsed macrophages

S. Z. Ben-Sasson, M. F. Lipscomb, T. F. Tucker, J. W. Uhr

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Peritoneal exudate lymphocytes (PEL) from immune guinea pigs that adhere to antigen-pulsed macrophages (M∅) were cultured for 1 week to yield a population enriched in antigen-specific (selected) T cells. These cells bind specifically within hours to fresh autologous antigen-pulsed M∅. The dissociation of these selected PEL from antigen-pulsed M∅ was studied. No evidence was obtained that factors in the culture medium play a role in dissociation. Lymphocytes that have dissociated from antigen-pulsed M∅ are usually fully capable of rebinding to M∅ freshly pulsed with antigen, suggesting that there is no deficiency in the lymphocytes ability to bind. In contrast, readding antigen to cultures during incubation prevents the predicted dissociation. Moreover, repulsing M∅ cultured without selected PEL restores their capacity to bind fresh selected PEL. These findings indicate that decay of antigen associated with M∅ is the major mechanism underlying the observed dissociation.

Original languageEnglish (US)
Pages (from-to)1902-1906
Number of pages5
JournalJournal of Immunology
Volume120
Issue number6
StatePublished - Dec 1 1978

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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