Specific patterns of Cdc42 activity are related to distinct elements of T cell polarization

Irina Tskvitaria-Fuller, Abhinav Seth, Neeta Mistry, Hua Gu, Michael K. Rosen, Christoph Wülfing

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

T cell polarization toward and within the cellular interface with an APC is critical for effective T cell activation. The Rho family GTPase Cdc42 is a central regulator of cellular polarization. Using live-cell imaging, we characterized the spatiotemporal patterns of Cdc42 activity and their physiological regulation. Using three independent means of experimental manipulation of Cdc42 activity, we established that Cdc42 is a critical regulator of T cell actin dynamics, TCR clustering, and cell cycle entry. Using quantification of three-dimensional data, we could relate distinct spatiotemporal patterns of Cdc42 activity to specific elements of T cell activation. This result suggests that Cdc42 activity in specific locations at specific times is most critical for its function in T cell activation.

Original languageEnglish (US)
Pages (from-to)1708-1720
Number of pages13
JournalJournal of Immunology
Volume177
Issue number3
StatePublished - Aug 1 2006

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T-Lymphocytes
rho GTP-Binding Proteins
Cluster Analysis
Actins
Cell Cycle

ASJC Scopus subject areas

  • Immunology

Cite this

Tskvitaria-Fuller, I., Seth, A., Mistry, N., Gu, H., Rosen, M. K., & Wülfing, C. (2006). Specific patterns of Cdc42 activity are related to distinct elements of T cell polarization. Journal of Immunology, 177(3), 1708-1720.

Specific patterns of Cdc42 activity are related to distinct elements of T cell polarization. / Tskvitaria-Fuller, Irina; Seth, Abhinav; Mistry, Neeta; Gu, Hua; Rosen, Michael K.; Wülfing, Christoph.

In: Journal of Immunology, Vol. 177, No. 3, 01.08.2006, p. 1708-1720.

Research output: Contribution to journalArticle

Tskvitaria-Fuller, I, Seth, A, Mistry, N, Gu, H, Rosen, MK & Wülfing, C 2006, 'Specific patterns of Cdc42 activity are related to distinct elements of T cell polarization', Journal of Immunology, vol. 177, no. 3, pp. 1708-1720.
Tskvitaria-Fuller I, Seth A, Mistry N, Gu H, Rosen MK, Wülfing C. Specific patterns of Cdc42 activity are related to distinct elements of T cell polarization. Journal of Immunology. 2006 Aug 1;177(3):1708-1720.
Tskvitaria-Fuller, Irina ; Seth, Abhinav ; Mistry, Neeta ; Gu, Hua ; Rosen, Michael K. ; Wülfing, Christoph. / Specific patterns of Cdc42 activity are related to distinct elements of T cell polarization. In: Journal of Immunology. 2006 ; Vol. 177, No. 3. pp. 1708-1720.
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