Specific patterns of Cdc42 activity are related to distinct elements of T cell polarization

Irina Tskvitaria-Fuller, Abhinav Seth, Neeta Mistry, Hua Gu, Michael K. Rosen, Christoph Wülfing

Research output: Contribution to journalArticlepeer-review

44 Scopus citations

Abstract

T cell polarization toward and within the cellular interface with an APC is critical for effective T cell activation. The Rho family GTPase Cdc42 is a central regulator of cellular polarization. Using live-cell imaging, we characterized the spatiotemporal patterns of Cdc42 activity and their physiological regulation. Using three independent means of experimental manipulation of Cdc42 activity, we established that Cdc42 is a critical regulator of T cell actin dynamics, TCR clustering, and cell cycle entry. Using quantification of three-dimensional data, we could relate distinct spatiotemporal patterns of Cdc42 activity to specific elements of T cell activation. This result suggests that Cdc42 activity in specific locations at specific times is most critical for its function in T cell activation.

Original languageEnglish (US)
Pages (from-to)1708-1720
Number of pages13
JournalJournal of Immunology
Volume177
Issue number3
DOIs
StatePublished - Aug 1 2006

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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