TY - JOUR
T1 - Spectrin couples cell shape, cortical tension, and hippo signaling in retinal epithelial morphogenesis
AU - Deng, Hua
AU - Yang, Limin
AU - Wen, Pei
AU - Lei, Huiyan
AU - Blount, Paul
AU - Pan, Duojia
N1 - Funding Information:
We thank A. Wodarz for providing PAR-3 antibody. We also thank T. Lecuit, T. Suzuki, R. Dubreuil, the Bloomington and Vienna Stock Centers, and the Developmental Studies Hybridoma Bank for reagents. This work was supported in part by grants from the National Institutes of Health (EY015708 to D. Pan and GM121780 to P. Blount) and the Welch Foundation (I-1420 to P. Blount). D. Pan is an investigator of the Howard Hughes Medical Institute.
Funding Information:
Vienna Stock Centers, and the Developmental Studies Hybridoma Bank for reagents. This work was supported in part by grants from the National Institutes of Health (EY015708 to D. Pan and GM121780 to P. Blount) and the Welch Foundation (I-1420 to P. Blount). D. Pan is an investigator of the Howard Hughes Medical Institute. The authors declare no competing financial interests.
Publisher Copyright:
© 2020 Deng et al.
PY - 2020
Y1 - 2020
N2 - Although extracellular force has a profound effect on cell shape, cytoskeleton tension, and cell proliferation through the Hippo signaling effector Yki/YAP/TAZ, how intracellular force regulates these processes remains poorly understood. Here, we report an essential role for spectrin in specifying cell shape by transmitting intracellular actomyosin force to cell membrane. While activation of myosin II in Drosophila melanogaster pupal retina leads to increased cortical tension, apical constriction, and Yki-mediated hyperplasia, spectrin mutant cells, despite showing myosin II activation and Yki-mediated hyperplasia, paradoxically display decreased cortical tension and expanded apical area. Mechanistically, we show that spectrin is required for tethering cortical F-actin to cell membrane domains outside the adherens junctions (AJs). Thus, in the absence of spectrin, the weakened attachment of cortical F-actin to plasma membrane results in a failure to transmit actomyosin force to cell membrane, causing an expansion of apical surfaces. These results uncover an essential mechanism that couples cell shape, cortical tension, and Hippo signaling and highlight the importance of non–AJ membrane domains in dictating cell shape in tissue morphogenesis.
AB - Although extracellular force has a profound effect on cell shape, cytoskeleton tension, and cell proliferation through the Hippo signaling effector Yki/YAP/TAZ, how intracellular force regulates these processes remains poorly understood. Here, we report an essential role for spectrin in specifying cell shape by transmitting intracellular actomyosin force to cell membrane. While activation of myosin II in Drosophila melanogaster pupal retina leads to increased cortical tension, apical constriction, and Yki-mediated hyperplasia, spectrin mutant cells, despite showing myosin II activation and Yki-mediated hyperplasia, paradoxically display decreased cortical tension and expanded apical area. Mechanistically, we show that spectrin is required for tethering cortical F-actin to cell membrane domains outside the adherens junctions (AJs). Thus, in the absence of spectrin, the weakened attachment of cortical F-actin to plasma membrane results in a failure to transmit actomyosin force to cell membrane, causing an expansion of apical surfaces. These results uncover an essential mechanism that couples cell shape, cortical tension, and Hippo signaling and highlight the importance of non–AJ membrane domains in dictating cell shape in tissue morphogenesis.
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U2 - 10.1083/JCB.201907018
DO - 10.1083/JCB.201907018
M3 - Article
C2 - 32328630
AN - SCOPUS:85084030641
VL - 219
JO - Journal of Cell Biology
JF - Journal of Cell Biology
SN - 0021-9525
IS - 4
M1 - e201907018
ER -