Spontaneous loss and subsequent stimulation of H-2 expression in clones of a heterozygous lymphoma cell line

Bodo Holtkamp, Kirsten Fischer Lindahl, Miriam Segall, Klaus Rajewsky

Research output: Contribution to journalArticle

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Abstract

The expression of H-2Kk antigens in a (C3H × DBA/2)F1 lymphoma cell line growing in vitro was investigated with monoclonal antibodies specific for a public antigen of the H-2Kk region (H-2.m3) in fluorescence analysis and microcytotoxicity assays and in cell-mediated cytotoxicity with allogeneically stimulated effector cells. Estimates of relative levels of H-2Kk-antigen expression obtained by the different methods were highly correlated. The uncloned, unselected population gradually lost H-2Kk surface antigen expression under culture conditions. This was due to the appearance of H-2Kk negative variants. Fifteen cloned sublines of a population enriched for cells expressing antigen H-2.m3 in the fluorescence activated cell sorter contained either two distinct populations, one consisting of H-2.m3 negative and one of H-2.m3 positive cells, or consisted of H-2.m3 negative cells only. The expression of the H-2.m3 determinant of H-2Kk paralleled that of other serological H-2Kk determinants and of H-2Kk target determinants for cell-mediated cytotoxicity. In nearly all clones where two populations could be detected, the proportion of H-2.m3 negative cells increased with time in culture. The amounts of H-2Kk antigen expressed by the clones appeared not to be correlated to the amounts of H-2Dk antigens on the cell surface as judged by cell-mediated cytotoxicity. In at least one clone and in the uncloned population, H-2Kk-antigen expression detectable by fluorescence analysis could be stimulated by growing the cells in the peritoneal cavities of (C3H × DBA/2)F1 mice or by adding mouse interferon preparations to the cell cultures. The increase in susceptibility to cell-mediated lympholysis of cells grown in vivo paralleled the increase in H-2 expression detected by fluorescence. In contrast, cells growing in the presence of interferon in vitro showed reduced sensitivity to lysis by alloreactive lymphocytes, although H-2 antigens were strongly expressed as measured by fluorescence.

Original languageEnglish (US)
Pages (from-to)405-421
Number of pages17
JournalImmunogenetics
Volume9
Issue number1
DOIs
StatePublished - Dec 1979

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Lymphoma
Clone Cells
Cell Line
Fluorescence
H-2 Antigens
Population
Interferons
Peritoneal Cavity
Surface Antigens
H antigen
Cell Culture Techniques
Monoclonal Antibodies
Lymphocytes

ASJC Scopus subject areas

  • Immunology
  • Genetics

Cite this

Spontaneous loss and subsequent stimulation of H-2 expression in clones of a heterozygous lymphoma cell line. / Holtkamp, Bodo; Lindahl, Kirsten Fischer; Segall, Miriam; Rajewsky, Klaus.

In: Immunogenetics, Vol. 9, No. 1, 12.1979, p. 405-421.

Research output: Contribution to journalArticle

Holtkamp, Bodo ; Lindahl, Kirsten Fischer ; Segall, Miriam ; Rajewsky, Klaus. / Spontaneous loss and subsequent stimulation of H-2 expression in clones of a heterozygous lymphoma cell line. In: Immunogenetics. 1979 ; Vol. 9, No. 1. pp. 405-421.
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