Spurious prostate-specific antigen (PSA) recurrence after radical prostatectomy: Interference by human antimouse heterophile antibodies

Sangtae Park, Frank H. Wians, Jeffrey A Cadeddu

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

Human antimouse heterophile antibodies (HAMA) are naturally occurring antibodies that can interfere with modern serum assays. We report a case of HAMA interference with a commonly used prostate-specific antigen (PSA) assay, leading to false elevation (2.17-2.46 ng/mL) after radical prostatectomy. Pre-operative PSA was 4.4 ng/mL, and final pathology was Gleason 3 + 3, pT2cNXMX. This markedly elevated postoperative PSA led to unnecessary imaging for metastasis and psychological distress to the patient. Direct measurement of HAMA in the patient's serum yielded a value of 440 ng/mL (<74 ng/mL). An alternate PSA assay using goat detection antibody eliminated interference, with all values 0.05 ng/mL. When a patient's PSA is inconsistent with the clinical scenario, one should consider immunological interference by HAMA in PSA assays.

Original languageEnglish (US)
Pages (from-to)251-253
Number of pages3
JournalInternational Journal of Urology
Volume14
Issue number3
DOIs
StatePublished - Mar 1 2007

Keywords

  • Biochemical recurrence
  • Heterophile antibody
  • Immunoassay
  • Prostate specific antigen

ASJC Scopus subject areas

  • Urology

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