TY - JOUR
T1 - Spurious prostate-specific antigen (PSA) recurrence after radical prostatectomy
T2 - Interference by human antimouse heterophile antibodies
AU - Park, Sangtae
AU - Wians, Frank H.
AU - Cadeddu, Jeffrey A
N1 - Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2007/3
Y1 - 2007/3
N2 - Human antimouse heterophile antibodies (HAMA) are naturally occurring antibodies that can interfere with modern serum assays. We report a case of HAMA interference with a commonly used prostate-specific antigen (PSA) assay, leading to false elevation (2.17-2.46 ng/mL) after radical prostatectomy. Pre-operative PSA was 4.4 ng/mL, and final pathology was Gleason 3 + 3, pT2cNXMX. This markedly elevated postoperative PSA led to unnecessary imaging for metastasis and psychological distress to the patient. Direct measurement of HAMA in the patient's serum yielded a value of 440 ng/mL (<74 ng/mL). An alternate PSA assay using goat detection antibody eliminated interference, with all values 0.05 ng/mL. When a patient's PSA is inconsistent with the clinical scenario, one should consider immunological interference by HAMA in PSA assays.
AB - Human antimouse heterophile antibodies (HAMA) are naturally occurring antibodies that can interfere with modern serum assays. We report a case of HAMA interference with a commonly used prostate-specific antigen (PSA) assay, leading to false elevation (2.17-2.46 ng/mL) after radical prostatectomy. Pre-operative PSA was 4.4 ng/mL, and final pathology was Gleason 3 + 3, pT2cNXMX. This markedly elevated postoperative PSA led to unnecessary imaging for metastasis and psychological distress to the patient. Direct measurement of HAMA in the patient's serum yielded a value of 440 ng/mL (<74 ng/mL). An alternate PSA assay using goat detection antibody eliminated interference, with all values 0.05 ng/mL. When a patient's PSA is inconsistent with the clinical scenario, one should consider immunological interference by HAMA in PSA assays.
KW - Biochemical recurrence
KW - Heterophile antibody
KW - Immunoassay
KW - Prostate specific antigen
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U2 - 10.1111/j.1442-2042.2006.01648.x
DO - 10.1111/j.1442-2042.2006.01648.x
M3 - Article
C2 - 17430267
AN - SCOPUS:33947274012
SN - 0919-8172
VL - 14
SP - 251
EP - 253
JO - International Journal of Urology
JF - International Journal of Urology
IS - 3
ER -