Stain is the major factor for chondrocyte death in articular cartilage under static load

Amit K. Shah, Li Jen Yuan, Peter A. Torzilli, C. T Christopher Chen

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Several studies have shown that stress, strain, and stress/strain rate at certain levels can kill chondrocytes, but the major factor is unclear. The objective of this study was to determine the effects of strain and stress on chondrocyte death. Bovine cartilage explants (5 mm) at the age of 2 and 24 month were indented (3 mm) at 3.12, 6.25 and 12.5 MPa to reach a final strain of 15, 30, 45, 60 and 75%. Cell death in the center and on the edges of the indented region was assessed at 0, 3 and 7 days post-loading, and quantified using commercially available software. Our results showed cell death in the center region increased with strain (p<0.001) but decreased with age (p<0.001). Cell death at the edges of the indented region was greater than that of the center (p<0.001), and increased with strain (p<0.001). With post-load incubation, a decrease of cell death was found in the explant loaded with 60 and 75%. Cells with positive TUNEL-staining and positive M30-staining were consistently seen in the middle and deep zones in the center region 3 days after load removal. This suggests that two types of cell injury/death (necrosis and apoptosis) occur simultaneously. Linear regression and ANOVA suggest that strain is the major factor for chondrocyte death under static load.

Original languageEnglish (US)
Title of host publicationAmerican Society of Mechanical Engineers, Bioengineering Division (Publication) BED
PublisherAmerican Society of Mechanical Engineers (ASME)
Pages331-332
Number of pages2
Volume55
Publication statusPublished - 2003
Event2003 ASME International Mechanical Engineering Congress - Washington, DC., United States
Duration: Nov 15 2003Nov 21 2003

Other

Other2003 ASME International Mechanical Engineering Congress
CountryUnited States
CityWashington, DC.
Period11/15/0311/21/03

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ASJC Scopus subject areas

  • Engineering(all)

Cite this

Shah, A. K., Yuan, L. J., Torzilli, P. A., & Chen, C. T. C. (2003). Stain is the major factor for chondrocyte death in articular cartilage under static load. In American Society of Mechanical Engineers, Bioengineering Division (Publication) BED (Vol. 55, pp. 331-332). American Society of Mechanical Engineers (ASME).