Staphylococcal enterotoxin D functions as a human B cell superantigen by rescuing VH4-expressing B cells from apoptosis

Rana Domiati-Saad, Jeanne F. Attrep, Hans Peter Brezinschek, Anne H. Cherrie, David R. Karp, Peter E. Lipsky

Research output: Contribution to journalArticle

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Abstract

Staphylococcal enterotoxins are potent superantigens, in that they activate T cells bearing specific Vβ-chain gene segments. In this study, we analyzed the capacity of staphylococcal enterotoxin D (SED) to function as a B cell superantigen. SED induced T cell-dependent polyclonal proliferation and differentiation of B cells. In the absence of T cells, SED induced survival of B cells uniquely expressing VH4 containing IgM. The mechanism of survival of VH4-expressing B cells appeared to relate to the countering of apoptosis initiated by the engagement of HLA-DR by SED. Analysis of the VH4 gene products expressed by SED-stimulated B cells revealed the usage of six of the known functional VH4 genes with a variety of different CDR3 regions, employing different DH and JH gene segments. Moreover, the sequence analysis identified a possible site for SED binding of VH4 that includes the solvent-exposed surfaces of 3′ CDR2/FR3 and/or FR1. Thus, SED appears to function as a unique B cell superantigen by inducing survival of VH4-expressing B cells.

Original languageEnglish (US)
Pages (from-to)3608-3620
Number of pages13
JournalJournal of Immunology
Volume156
Issue number10
StatePublished - May 15 1996

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Superantigens
B-Lymphocytes
Apoptosis
T-Lymphocytes
Genes
Survival
Enterotoxins
HLA-DR Antigens
Staphylococcal enterotoxin D
Immunoglobulin M
Sequence Analysis

ASJC Scopus subject areas

  • Immunology

Cite this

Domiati-Saad, R., Attrep, J. F., Brezinschek, H. P., Cherrie, A. H., Karp, D. R., & Lipsky, P. E. (1996). Staphylococcal enterotoxin D functions as a human B cell superantigen by rescuing VH4-expressing B cells from apoptosis. Journal of Immunology, 156(10), 3608-3620.

Staphylococcal enterotoxin D functions as a human B cell superantigen by rescuing VH4-expressing B cells from apoptosis. / Domiati-Saad, Rana; Attrep, Jeanne F.; Brezinschek, Hans Peter; Cherrie, Anne H.; Karp, David R.; Lipsky, Peter E.

In: Journal of Immunology, Vol. 156, No. 10, 15.05.1996, p. 3608-3620.

Research output: Contribution to journalArticle

Domiati-Saad, R, Attrep, JF, Brezinschek, HP, Cherrie, AH, Karp, DR & Lipsky, PE 1996, 'Staphylococcal enterotoxin D functions as a human B cell superantigen by rescuing VH4-expressing B cells from apoptosis', Journal of Immunology, vol. 156, no. 10, pp. 3608-3620.
Domiati-Saad R, Attrep JF, Brezinschek HP, Cherrie AH, Karp DR, Lipsky PE. Staphylococcal enterotoxin D functions as a human B cell superantigen by rescuing VH4-expressing B cells from apoptosis. Journal of Immunology. 1996 May 15;156(10):3608-3620.
Domiati-Saad, Rana ; Attrep, Jeanne F. ; Brezinschek, Hans Peter ; Cherrie, Anne H. ; Karp, David R. ; Lipsky, Peter E. / Staphylococcal enterotoxin D functions as a human B cell superantigen by rescuing VH4-expressing B cells from apoptosis. In: Journal of Immunology. 1996 ; Vol. 156, No. 10. pp. 3608-3620.
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