Starvation activates MAP kinase through the muscarinic acetylcholine pathway in Caenorhabditis elegans pharynx

Young Jai You; Jeongho Kim; Melanie Cobb; Leon Avery

doi:10.1016/j.cmet.2006.02.012

Starvation activates MAP kinase through the muscarinic acetylcholine pathway in Caenorhabditis elegans pharynx

Young Jai You, Jeongho Kim, Melanie Cobb, Leon Avery

Research output: Contribution to journal › Article › peer-review

76 Scopus citations

Abstract

Starvation activates MAPK in the pharyngeal muscles of C. elegans through a muscarinic acetylcholine receptor, G_qα, and nPKC as shown by the following results: (1) Starvation causes phosphorylation of MAPK in pharyngeal muscle. (2) In a sensitized genetic background in which G_qα signaling cannot be downregulated, activation of the pathway by a muscarinic agonist causes lethal changes in pharyngeal muscle function. Starvation has identical effects. (3) A muscarinic antagonist blocks the effects of starvation on sensitized muscle. (4) Mutations and drugs that block any step of signaling from the muscarinic receptor to MAPK also block the effects of starvation on sensitized muscle. (5) Overexpression of MAPK in wild-type pharyngeal muscle mimics the effects of muscarinic agonist and of starvation on sensitized muscle. We suggest that, during starvation, the muscarinic pathway to MAPK is activated to change the pharyngeal muscle physiology to enhance ingestion of food when food becomes available.

Original language	English (US)
Pages (from-to)	237-245
Number of pages	9
Journal	Cell Metabolism
Volume	3
Issue number	4
DOIs	https://doi.org/10.1016/j.cmet.2006.02.012
State	Published - Apr 2006

ASJC Scopus subject areas

Physiology
Molecular Biology
Cell Biology

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title = "Starvation activates MAP kinase through the muscarinic acetylcholine pathway in Caenorhabditis elegans pharynx",

abstract = "Starvation activates MAPK in the pharyngeal muscles of C. elegans through a muscarinic acetylcholine receptor, Gqα, and nPKC as shown by the following results: (1) Starvation causes phosphorylation of MAPK in pharyngeal muscle. (2) In a sensitized genetic background in which Gqα signaling cannot be downregulated, activation of the pathway by a muscarinic agonist causes lethal changes in pharyngeal muscle function. Starvation has identical effects. (3) A muscarinic antagonist blocks the effects of starvation on sensitized muscle. (4) Mutations and drugs that block any step of signaling from the muscarinic receptor to MAPK also block the effects of starvation on sensitized muscle. (5) Overexpression of MAPK in wild-type pharyngeal muscle mimics the effects of muscarinic agonist and of starvation on sensitized muscle. We suggest that, during starvation, the muscarinic pathway to MAPK is activated to change the pharyngeal muscle physiology to enhance ingestion of food when food becomes available.",

author = "You, {Young Jai} and Jeongho Kim and Melanie Cobb and Leon Avery",

note = "Funding Information: We thank Kate Steger for helpful discussion and strains, Daniel Omura for a GFP expressing E. coli strain, Yuji Kohara for providing the mpk-1 cDNA and Dr. Min Han for DNA constructs. Some nematode strains used in this work were provided by the Caenorhabditis Genetics Center, which is funded by the NIH National Center for Research Resources (NCRR). This research was supported by National Institutes of Health research grants HL46154 (L.A.) and DK34128 (M.H.C) and Korea Research Foundation Grant KRF-2005-070-C00118 (J.K.). ",

year = "2006",

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doi = "10.1016/j.cmet.2006.02.012",

language = "English (US)",

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AU - You, Young Jai

AU - Kim, Jeongho

AU - Cobb, Melanie

AU - Avery, Leon

N1 - Funding Information: We thank Kate Steger for helpful discussion and strains, Daniel Omura for a GFP expressing E. coli strain, Yuji Kohara for providing the mpk-1 cDNA and Dr. Min Han for DNA constructs. Some nematode strains used in this work were provided by the Caenorhabditis Genetics Center, which is funded by the NIH National Center for Research Resources (NCRR). This research was supported by National Institutes of Health research grants HL46154 (L.A.) and DK34128 (M.H.C) and Korea Research Foundation Grant KRF-2005-070-C00118 (J.K.).

PY - 2006/4

Y1 - 2006/4

N2 - Starvation activates MAPK in the pharyngeal muscles of C. elegans through a muscarinic acetylcholine receptor, Gqα, and nPKC as shown by the following results: (1) Starvation causes phosphorylation of MAPK in pharyngeal muscle. (2) In a sensitized genetic background in which Gqα signaling cannot be downregulated, activation of the pathway by a muscarinic agonist causes lethal changes in pharyngeal muscle function. Starvation has identical effects. (3) A muscarinic antagonist blocks the effects of starvation on sensitized muscle. (4) Mutations and drugs that block any step of signaling from the muscarinic receptor to MAPK also block the effects of starvation on sensitized muscle. (5) Overexpression of MAPK in wild-type pharyngeal muscle mimics the effects of muscarinic agonist and of starvation on sensitized muscle. We suggest that, during starvation, the muscarinic pathway to MAPK is activated to change the pharyngeal muscle physiology to enhance ingestion of food when food becomes available.

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Starvation activates MAP kinase through the muscarinic acetylcholine pathway in Caenorhabditis elegans pharynx

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