Steroid-producing cells regulate arterial tone of adrenal cortical arteries

David X. Zhang, Kathryn M. Gauthier, J R Falck, Anjaiah Siddam, William B. Campbell

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Adrenal blood flow is coupled to adrenal hormone secretion. ACTH increases adrenal blood flow and stimulates the secretion of aldosterone and cortisol in vivo. However, ACTH does not alter vascular tone of isolated adrenal cortical arteries. Mechanisms underlying this discrepancy remain unsolved. The present study examined the effect of zona glomerulosa (ZG) cells on cortical arterial tone. ZG cells (105 to 107 cells) and ZG cell-conditioned medium relaxed preconstricted adrenal arteries (maximal relaxations = 79 ± 4 and 66 ± 4%, respectively). In adrenal arteries coincubated with a small number of ZG cells (0.5-1 × 106), ACTH (10 -12 to 10-8 M) induced concentration-dependent relaxations (maximal relaxation = 67 ± 4%). Similarly, ACTH (10-8 M) dilated (55 ± 10%) perfused arteries embedded in adrenal cortical slices. ZG cell-dependent relaxations to ACTH were endothelium-independent and inhibited by high extracellular K+ (60 mM); the K+ channel blocker, iberiotoxin (100 nM); the cytochrome P450 inhibitors SKF 525A (10 μM) and miconazole (10 μM); and the epoxyeicosatrienoic acid (EET) antagonist 14,15-EEZE (2 μM). Four EET regioisomers were identified in ZG cell-conditioned media. EET production was stimulated by ACTH. We conclude that ZG cells release EETs and this release is stimulated by ACTH. Interaction of endocrine and vascular cells represents a mechanism for regulating adrenal blood flow and couples steroidogenesis to increased blood flow.

Original languageEnglish (US)
Pages (from-to)3569-3576
Number of pages8
JournalEndocrinology
Volume148
Issue number8
DOIs
StatePublished - Aug 2007

Fingerprint

Zona Glomerulosa
Adrenocorticotropic Hormone
Arteries
Steroids
Conditioned Culture Medium
Acids
Blood Vessels
Proadifen
Miconazole
Endocrine Cells
Aldosterone
Cytochrome P-450 Enzyme System
Endothelium
Hydrocortisone
Hormones

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Zhang, D. X., Gauthier, K. M., Falck, J. R., Siddam, A., & Campbell, W. B. (2007). Steroid-producing cells regulate arterial tone of adrenal cortical arteries. Endocrinology, 148(8), 3569-3576. https://doi.org/10.1210/en.2007-0169

Steroid-producing cells regulate arterial tone of adrenal cortical arteries. / Zhang, David X.; Gauthier, Kathryn M.; Falck, J R; Siddam, Anjaiah; Campbell, William B.

In: Endocrinology, Vol. 148, No. 8, 08.2007, p. 3569-3576.

Research output: Contribution to journalArticle

Zhang, DX, Gauthier, KM, Falck, JR, Siddam, A & Campbell, WB 2007, 'Steroid-producing cells regulate arterial tone of adrenal cortical arteries', Endocrinology, vol. 148, no. 8, pp. 3569-3576. https://doi.org/10.1210/en.2007-0169
Zhang, David X. ; Gauthier, Kathryn M. ; Falck, J R ; Siddam, Anjaiah ; Campbell, William B. / Steroid-producing cells regulate arterial tone of adrenal cortical arteries. In: Endocrinology. 2007 ; Vol. 148, No. 8. pp. 3569-3576.
@article{7d3d858de06f41a786f9f11ba1728908,
title = "Steroid-producing cells regulate arterial tone of adrenal cortical arteries",
abstract = "Adrenal blood flow is coupled to adrenal hormone secretion. ACTH increases adrenal blood flow and stimulates the secretion of aldosterone and cortisol in vivo. However, ACTH does not alter vascular tone of isolated adrenal cortical arteries. Mechanisms underlying this discrepancy remain unsolved. The present study examined the effect of zona glomerulosa (ZG) cells on cortical arterial tone. ZG cells (105 to 107 cells) and ZG cell-conditioned medium relaxed preconstricted adrenal arteries (maximal relaxations = 79 ± 4 and 66 ± 4{\%}, respectively). In adrenal arteries coincubated with a small number of ZG cells (0.5-1 × 106), ACTH (10 -12 to 10-8 M) induced concentration-dependent relaxations (maximal relaxation = 67 ± 4{\%}). Similarly, ACTH (10-8 M) dilated (55 ± 10{\%}) perfused arteries embedded in adrenal cortical slices. ZG cell-dependent relaxations to ACTH were endothelium-independent and inhibited by high extracellular K+ (60 mM); the K+ channel blocker, iberiotoxin (100 nM); the cytochrome P450 inhibitors SKF 525A (10 μM) and miconazole (10 μM); and the epoxyeicosatrienoic acid (EET) antagonist 14,15-EEZE (2 μM). Four EET regioisomers were identified in ZG cell-conditioned media. EET production was stimulated by ACTH. We conclude that ZG cells release EETs and this release is stimulated by ACTH. Interaction of endocrine and vascular cells represents a mechanism for regulating adrenal blood flow and couples steroidogenesis to increased blood flow.",
author = "Zhang, {David X.} and Gauthier, {Kathryn M.} and Falck, {J R} and Anjaiah Siddam and Campbell, {William B.}",
year = "2007",
month = "8",
doi = "10.1210/en.2007-0169",
language = "English (US)",
volume = "148",
pages = "3569--3576",
journal = "Endocrinology",
issn = "0013-7227",
publisher = "The Endocrine Society",
number = "8",

}

TY - JOUR

T1 - Steroid-producing cells regulate arterial tone of adrenal cortical arteries

AU - Zhang, David X.

AU - Gauthier, Kathryn M.

AU - Falck, J R

AU - Siddam, Anjaiah

AU - Campbell, William B.

PY - 2007/8

Y1 - 2007/8

N2 - Adrenal blood flow is coupled to adrenal hormone secretion. ACTH increases adrenal blood flow and stimulates the secretion of aldosterone and cortisol in vivo. However, ACTH does not alter vascular tone of isolated adrenal cortical arteries. Mechanisms underlying this discrepancy remain unsolved. The present study examined the effect of zona glomerulosa (ZG) cells on cortical arterial tone. ZG cells (105 to 107 cells) and ZG cell-conditioned medium relaxed preconstricted adrenal arteries (maximal relaxations = 79 ± 4 and 66 ± 4%, respectively). In adrenal arteries coincubated with a small number of ZG cells (0.5-1 × 106), ACTH (10 -12 to 10-8 M) induced concentration-dependent relaxations (maximal relaxation = 67 ± 4%). Similarly, ACTH (10-8 M) dilated (55 ± 10%) perfused arteries embedded in adrenal cortical slices. ZG cell-dependent relaxations to ACTH were endothelium-independent and inhibited by high extracellular K+ (60 mM); the K+ channel blocker, iberiotoxin (100 nM); the cytochrome P450 inhibitors SKF 525A (10 μM) and miconazole (10 μM); and the epoxyeicosatrienoic acid (EET) antagonist 14,15-EEZE (2 μM). Four EET regioisomers were identified in ZG cell-conditioned media. EET production was stimulated by ACTH. We conclude that ZG cells release EETs and this release is stimulated by ACTH. Interaction of endocrine and vascular cells represents a mechanism for regulating adrenal blood flow and couples steroidogenesis to increased blood flow.

AB - Adrenal blood flow is coupled to adrenal hormone secretion. ACTH increases adrenal blood flow and stimulates the secretion of aldosterone and cortisol in vivo. However, ACTH does not alter vascular tone of isolated adrenal cortical arteries. Mechanisms underlying this discrepancy remain unsolved. The present study examined the effect of zona glomerulosa (ZG) cells on cortical arterial tone. ZG cells (105 to 107 cells) and ZG cell-conditioned medium relaxed preconstricted adrenal arteries (maximal relaxations = 79 ± 4 and 66 ± 4%, respectively). In adrenal arteries coincubated with a small number of ZG cells (0.5-1 × 106), ACTH (10 -12 to 10-8 M) induced concentration-dependent relaxations (maximal relaxation = 67 ± 4%). Similarly, ACTH (10-8 M) dilated (55 ± 10%) perfused arteries embedded in adrenal cortical slices. ZG cell-dependent relaxations to ACTH were endothelium-independent and inhibited by high extracellular K+ (60 mM); the K+ channel blocker, iberiotoxin (100 nM); the cytochrome P450 inhibitors SKF 525A (10 μM) and miconazole (10 μM); and the epoxyeicosatrienoic acid (EET) antagonist 14,15-EEZE (2 μM). Four EET regioisomers were identified in ZG cell-conditioned media. EET production was stimulated by ACTH. We conclude that ZG cells release EETs and this release is stimulated by ACTH. Interaction of endocrine and vascular cells represents a mechanism for regulating adrenal blood flow and couples steroidogenesis to increased blood flow.

UR - http://www.scopus.com/inward/record.url?scp=34547098836&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34547098836&partnerID=8YFLogxK

U2 - 10.1210/en.2007-0169

DO - 10.1210/en.2007-0169

M3 - Article

C2 - 17446179

AN - SCOPUS:34547098836

VL - 148

SP - 3569

EP - 3576

JO - Endocrinology

JF - Endocrinology

SN - 0013-7227

IS - 8

ER -