Stimulation of γ globulin synthesis and specific antibody production by a factor released by activated lymphocytes

M. Rosenthal, P. Stastny, M. Ziff

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12 Scopus citations


By using a radioactive immune coprecipitation technique, supernatants of lymph node cells from immunized rabbits incubated with keyhole limpet hemocyanin (KLH) were shown to enhance γ globulin synthesis and anti KLH antibody production of KLH primed and nonprimed lymphoid cells from the peripheral blood and lymph nodes in the presence of KLH in vitro. Similarly, when KLH stimulated supernatants were added both to BSA primed and nonprimed lymphocyte cultures in the presence of BSA, increases in γ globulin and anti BSA synthesis were observed. The data presented provide evidence that a soluble factor is released by antigen stimulated lymphocytes which enhances immunoglobulin and specific antibody production in a nonspecific manner. The evidence obtained suggests that the factor may be a lymphokine which either directly or indirectly stimulates bone marrow derived lymphocyte function in both the primary and secondary responses.

Original languageEnglish (US)
Pages (from-to)1119-1127
Number of pages9
JournalJournal of Immunology
Issue number4
Publication statusPublished - 1973


ASJC Scopus subject areas

  • Immunology

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