Structural basis for conserved complement factor-like function in the antimalarial protein TEP1

Richard H G Baxter, Chung I. Chang, Yogarany Chelliah, Stéphanie Blandin, Elena A. Levashina, Johann Deisenhofer

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

Thioester-containing proteins (TEPs) are a major component of the innate immune response of insects to invasion by bacteria and protozoa. TEPs form a distinct clade of a superfamily that includes the pan-protease inhibitors α2-macroglobulins and vertebrate complement factors. The essential feature of these proteins is a sequestered thioester bond that, after cleavage in a protease-sensitive region of the protein, is activated and covalently binds to its target. Recently, TEP1 from the malarial vector Anopheles gambiae was shown to mediate recognition and killing of ookinetes from the malarial parasite Plasmodium berghei, a model for the human malarial parasite Plasmodium falciparum. Here, we present the crystal structure of the TEP1 isoform TEP1r. Although the overall protein fold of TEP1r resembles that of complement factor C3, the TEP1r domains are repositioned to stabilize the inactive conformation of the molecule (containing an intact thioester) in the absence of the anaphylotoxin domain, a central component of complement factors. The structure of TEP1r provides a molecular basis for the differences between TEP1 alleles TEP1r and TEP1s, which correlate with resistance of A. gambiae to infection by P. berghei.

Original languageEnglish (US)
Pages (from-to)11615-11620
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume104
Issue number28
DOIs
StatePublished - Jul 10 2007

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Antimalarials
Anopheles gambiae
Plasmodium berghei
Proteins
Parasites
Macroglobulins
Complement C3
Plasmodium falciparum
Protease Inhibitors
Innate Immunity
Insects
Vertebrates
Protein Isoforms
Peptide Hydrolases
Alleles
Bacteria
Infection

Keywords

  • Anopheles gambiae
  • Crystal structure
  • Innate immunity
  • Thioester

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Structural basis for conserved complement factor-like function in the antimalarial protein TEP1. / Baxter, Richard H G; Chang, Chung I.; Chelliah, Yogarany; Blandin, Stéphanie; Levashina, Elena A.; Deisenhofer, Johann.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, No. 28, 10.07.2007, p. 11615-11620.

Research output: Contribution to journalArticle

Baxter, Richard H G ; Chang, Chung I. ; Chelliah, Yogarany ; Blandin, Stéphanie ; Levashina, Elena A. ; Deisenhofer, Johann. / Structural basis for conserved complement factor-like function in the antimalarial protein TEP1. In: Proceedings of the National Academy of Sciences of the United States of America. 2007 ; Vol. 104, No. 28. pp. 11615-11620.
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