Structural basis of multifunctional bovine mitochondrial cytochrome bc1 complex

Chang An Yu, Hua Tian, Li Zhang, Kai Ping Deng, Sudha K. Shenoy, Linda Yu, Di Xia, Hoeon Kim, Johann Deisenhofer

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

The mitochondrial cytochrome bc1 complex is a multifunctional membrane protein complex. It catalyzes electron transfer, proton translocation, peptide processing, and superoxide generation. Crystal structure data at 2.9 Å resolution not only establishes the location of the redox centers and inhibitor binding sites, but also suggests a movement of the head domain of the iron-sulfur protein (ISP) during bc1 catalysis and inhibition of peptide-processing activity during complex maturation. The functional importance of the movement of extramembrane (head) domain of ISP in the bc1 complex is confirmed by analysis of the Rhodobacter sphaeroides bc1 complex mutants with increased rigidity in the ISP neck and by the determination of rate constants for acid/base-induced intramolecular electron transfer between [2Fe-2S] and heme c1 in native and inhibitor-loaded beef complexes. The peptide-processing activity is activated in bovine heart mitochondrial bc1 complex by nonionic detergent at concentrations that inactivate electron transfer activity. This peptide-processing activity is shown to be associated with subunits I and II by cloning, overexpression and in vitro reconstitution. The superoxide-generation site of the cytochrome bc1 complex is located at reduced b(L) and Q(·-). The reaction is membrane potential-, and cytochrome c-dependent.

Original languageEnglish (US)
Pages (from-to)191-199
Number of pages9
JournalJournal of Bioenergetics and Biomembranes
Volume31
Issue number3
DOIs
StatePublished - 1999

Fingerprint

Electron Transport Complex III
Iron-Sulfur Proteins
Head Movements
Peptides
Electrons
Superoxides
Rhodobacter sphaeroides
Cytochromes c
Catalysis
Heme
Detergents
Membrane Potentials
Oxidation-Reduction
Protons
Organism Cloning
Membrane Proteins
Neck
Binding Sites
Acids

Keywords

  • Cytochrome bc complex
  • Electron transfer inhibitors
  • Electron transfer reaction
  • Mitochondrial- processing peptidase
  • Superoxide-generation activity

ASJC Scopus subject areas

  • Physiology
  • Cell Biology

Cite this

Structural basis of multifunctional bovine mitochondrial cytochrome bc1 complex. / Yu, Chang An; Tian, Hua; Zhang, Li; Deng, Kai Ping; Shenoy, Sudha K.; Yu, Linda; Xia, Di; Kim, Hoeon; Deisenhofer, Johann.

In: Journal of Bioenergetics and Biomembranes, Vol. 31, No. 3, 1999, p. 191-199.

Research output: Contribution to journalArticle

Yu, Chang An ; Tian, Hua ; Zhang, Li ; Deng, Kai Ping ; Shenoy, Sudha K. ; Yu, Linda ; Xia, Di ; Kim, Hoeon ; Deisenhofer, Johann. / Structural basis of multifunctional bovine mitochondrial cytochrome bc1 complex. In: Journal of Bioenergetics and Biomembranes. 1999 ; Vol. 31, No. 3. pp. 191-199.
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