Structural changes in glycogen phosphorylase induced by phosphorylation

S. R. Sprang, K. R. Acharya, E. J. Goldsmith, D. I. Stuart, K. Varvill, R. J. Fletterick, N. B. Madsen, L. N. Johnson

Research output: Contribution to journalArticle

225 Citations (Scopus)

Abstract

A comparison of the refined crystal structures of dimeric glycogen phosphorylase b and a reveals structural changes that represent the first step in the activation of the enzyme. On phosphorylation of serine-14, the N-terminus of each subunit assumes an ordered helical conformation and binds to the surface of the dimer. The consequent structural changes at the N- and C-terminal regions lead to strengthened interactions between subunits and alter the binding sites for allosteric effectors and substrates.

Original languageEnglish (US)
Pages (from-to)215-221
Number of pages7
JournalNature
Volume336
Issue number6196
StatePublished - 1988

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Phosphorylase b
Phosphorylase a
Glycogen Phosphorylase
Enzyme Activation
Serine
Binding Sites
Phosphorylation

ASJC Scopus subject areas

  • General

Cite this

Sprang, S. R., Acharya, K. R., Goldsmith, E. J., Stuart, D. I., Varvill, K., Fletterick, R. J., ... Johnson, L. N. (1988). Structural changes in glycogen phosphorylase induced by phosphorylation. Nature, 336(6196), 215-221.

Structural changes in glycogen phosphorylase induced by phosphorylation. / Sprang, S. R.; Acharya, K. R.; Goldsmith, E. J.; Stuart, D. I.; Varvill, K.; Fletterick, R. J.; Madsen, N. B.; Johnson, L. N.

In: Nature, Vol. 336, No. 6196, 1988, p. 215-221.

Research output: Contribution to journalArticle

Sprang, SR, Acharya, KR, Goldsmith, EJ, Stuart, DI, Varvill, K, Fletterick, RJ, Madsen, NB & Johnson, LN 1988, 'Structural changes in glycogen phosphorylase induced by phosphorylation', Nature, vol. 336, no. 6196, pp. 215-221.
Sprang SR, Acharya KR, Goldsmith EJ, Stuart DI, Varvill K, Fletterick RJ et al. Structural changes in glycogen phosphorylase induced by phosphorylation. Nature. 1988;336(6196):215-221.
Sprang, S. R. ; Acharya, K. R. ; Goldsmith, E. J. ; Stuart, D. I. ; Varvill, K. ; Fletterick, R. J. ; Madsen, N. B. ; Johnson, L. N. / Structural changes in glycogen phosphorylase induced by phosphorylation. In: Nature. 1988 ; Vol. 336, No. 6196. pp. 215-221.
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