Structural studies on the murine ia alloantigens-III. Tryptic peptide comparisons of allelic products of the I-E/C sub-region

Richard G. Cook, Ellen S. Vitetta, Jonathan W. Uhr, J. Donald Capra

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Splenocytes from B10 congenic mice of the k, p, d, and r haplotypes were radiolabeled with 3H- or 14C-arginine, lysine, leucine and tyrosine. The E/C sub-region products were precipitated from NP40 lysates with specific alloantisera and the la α and β sub-units of each of the above four haplotypes were isolated by gel electrophoresis. Molecules to be compared, one 3H-labeled and the other 14C-labeled, were mixed, digested with trypsin, and the acid soluble peptides analyzed by ion-exchange chromatography. The results indicate that the α sub-units of p, d and r are approximately 90% homologous to k. In contrast, β polypeptides of the above three haplotypes have only about 50% coincidence of peptides with the k allelic product. This degree of structural variation suggests that the β sub-unit of the E/C product is encoded by the major histocompatibility complex.

Original languageEnglish (US)
Pages (from-to)29-35
Number of pages7
JournalMolecular Immunology
Issue number1
StatePublished - Jan 1979


ASJC Scopus subject areas

  • Immunology
  • Molecular Biology

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