Structural studies on the murine Ia alloantigens. IV. NH 2-terminal sequence analysis of allelic products of the I-A and I-E/C subregions

R. G. Cook, M. H. Siegelman, J. D. Capra, J. W. Uhr, E. S. Vitetta

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Murine Ia alloantigens encoded by the I-A and I-E/C subregions were isolated from radiolabeled splenic lysates and examined by NH 2-terminal sequence analysis. Haplotype-associated sequence variation was detected in the β, but not α, subunits of both the A and E/C alloantigens. The A β-polypeptides from k and b haplotypes show four differences in the 12 positions compared, whereas the E/C β-polypeptides from k and r haplotypes show two differences in the 13 positions compared. No sequence variation was detected between the A(k) and A(b) α-chains (six positions compared) or between the E/C(k) and E/C(r) α-chains (11 positions compared). Homology relationships between these murine Ia alloantigens and the human Ia (DR) alloantigens are also presented.

Original languageEnglish (US)
Pages (from-to)2232-2237
Number of pages6
JournalJournal of Immunology
Volume122
Issue number6
StatePublished - 1979

Fingerprint

Isoantigens
Sequence Analysis
Haplotypes
Peptides

ASJC Scopus subject areas

  • Immunology

Cite this

Structural studies on the murine Ia alloantigens. IV. NH 2-terminal sequence analysis of allelic products of the I-A and I-E/C subregions. / Cook, R. G.; Siegelman, M. H.; Capra, J. D.; Uhr, J. W.; Vitetta, E. S.

In: Journal of Immunology, Vol. 122, No. 6, 1979, p. 2232-2237.

Research output: Contribution to journalArticle

@article{cd96ed52f9fc48e1a352380dbc250ba0,
title = "Structural studies on the murine Ia alloantigens. IV. NH 2-terminal sequence analysis of allelic products of the I-A and I-E/C subregions",
abstract = "Murine Ia alloantigens encoded by the I-A and I-E/C subregions were isolated from radiolabeled splenic lysates and examined by NH 2-terminal sequence analysis. Haplotype-associated sequence variation was detected in the β, but not α, subunits of both the A and E/C alloantigens. The A β-polypeptides from k and b haplotypes show four differences in the 12 positions compared, whereas the E/C β-polypeptides from k and r haplotypes show two differences in the 13 positions compared. No sequence variation was detected between the A(k) and A(b) α-chains (six positions compared) or between the E/C(k) and E/C(r) α-chains (11 positions compared). Homology relationships between these murine Ia alloantigens and the human Ia (DR) alloantigens are also presented.",
author = "Cook, {R. G.} and Siegelman, {M. H.} and Capra, {J. D.} and Uhr, {J. W.} and Vitetta, {E. S.}",
year = "1979",
language = "English (US)",
volume = "122",
pages = "2232--2237",
journal = "Journal of Immunology",
issn = "0022-1767",
publisher = "American Association of Immunologists",
number = "6",

}

TY - JOUR

T1 - Structural studies on the murine Ia alloantigens. IV. NH 2-terminal sequence analysis of allelic products of the I-A and I-E/C subregions

AU - Cook, R. G.

AU - Siegelman, M. H.

AU - Capra, J. D.

AU - Uhr, J. W.

AU - Vitetta, E. S.

PY - 1979

Y1 - 1979

N2 - Murine Ia alloantigens encoded by the I-A and I-E/C subregions were isolated from radiolabeled splenic lysates and examined by NH 2-terminal sequence analysis. Haplotype-associated sequence variation was detected in the β, but not α, subunits of both the A and E/C alloantigens. The A β-polypeptides from k and b haplotypes show four differences in the 12 positions compared, whereas the E/C β-polypeptides from k and r haplotypes show two differences in the 13 positions compared. No sequence variation was detected between the A(k) and A(b) α-chains (six positions compared) or between the E/C(k) and E/C(r) α-chains (11 positions compared). Homology relationships between these murine Ia alloantigens and the human Ia (DR) alloantigens are also presented.

AB - Murine Ia alloantigens encoded by the I-A and I-E/C subregions were isolated from radiolabeled splenic lysates and examined by NH 2-terminal sequence analysis. Haplotype-associated sequence variation was detected in the β, but not α, subunits of both the A and E/C alloantigens. The A β-polypeptides from k and b haplotypes show four differences in the 12 positions compared, whereas the E/C β-polypeptides from k and r haplotypes show two differences in the 13 positions compared. No sequence variation was detected between the A(k) and A(b) α-chains (six positions compared) or between the E/C(k) and E/C(r) α-chains (11 positions compared). Homology relationships between these murine Ia alloantigens and the human Ia (DR) alloantigens are also presented.

UR - http://www.scopus.com/inward/record.url?scp=0018765074&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0018765074&partnerID=8YFLogxK

M3 - Article

C2 - 448125

AN - SCOPUS:0018765074

VL - 122

SP - 2232

EP - 2237

JO - Journal of Immunology

JF - Journal of Immunology

SN - 0022-1767

IS - 6

ER -