Structure of a Yeast 40S-eIF1-eIF1A-eIF3-eIF3j initiation complex

Christopher H.S. Aylett, Daniel Boehringer, Jan P. Erzberger, Tanja Schaefer, Nenad Ban

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

Eukaryotic translation initiation requires cooperative assembly of a large protein complex at the 40S ribosomal subunit. We have resolved a budding yeast initiation complex by cryo-EM, allowing placement of prior structures of eIF1, eIF1A, eIF3a, eIF3b and eIF3c. Our structure highlights differences in initiation-complex binding to the ribosome compared to that of mammalian eIF3, demonstrates a direct contact between eIF3j and eIF1A and reveals the network of interactions between eIF3 subunits.

Original languageEnglish (US)
Pages (from-to)269-271
Number of pages3
JournalNature Structural and Molecular Biology
Volume22
Issue number3
DOIs
StatePublished - Jan 1 2015

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Eukaryotic Small Ribosome Subunits
Saccharomycetales
Ribosomes
Yeasts
Proteins

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

Cite this

Structure of a Yeast 40S-eIF1-eIF1A-eIF3-eIF3j initiation complex. / Aylett, Christopher H.S.; Boehringer, Daniel; Erzberger, Jan P.; Schaefer, Tanja; Ban, Nenad.

In: Nature Structural and Molecular Biology, Vol. 22, No. 3, 01.01.2015, p. 269-271.

Research output: Contribution to journalArticle

Aylett, Christopher H.S. ; Boehringer, Daniel ; Erzberger, Jan P. ; Schaefer, Tanja ; Ban, Nenad. / Structure of a Yeast 40S-eIF1-eIF1A-eIF3-eIF3j initiation complex. In: Nature Structural and Molecular Biology. 2015 ; Vol. 22, No. 3. pp. 269-271.
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