Structure of the gene V protein of bacteriophage f1 determined by multiwavelength x-ray diffraction on the selenomethionyl protein

Matthew M. Skinner, Hong Zhang, Dale H. Leschnitzer, Yue Guan, Henry Bellamy, Robert M. Sweet, Carla W. Gray, Ruud N H Konings, Andrew H J Wang, Thomas C. Terwilliger

Research output: Contribution to journalArticle

110 Citations (Scopus)

Abstract

The crystal structure of the dimeric gene V protein of bacteriophage f1 was determined using multiwavelength anomalous diffraction on the selenomethionine-containing wild-type and isoleucine-47 → methionine mutant proteins with x-ray diffraction data phased to 2.5 Å resolution. The structure of the wild-type protein has been refined to an R factor of 19.2% using native data to 1.8 Å resolution. The structure of the gene V protein was used to obtain a model for the protein portion of the gene V protein- single-stranded DNA complex.

Original languageEnglish (US)
Pages (from-to)2071-2075
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume91
Issue number6
StatePublished - Mar 15 1994

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X-Rays
Proteins
Selenomethionine
Isoleucine
R Factors
Single-Stranded DNA
Mutant Proteins
Methionine
Enterobacteria phage f1 gene V protein

Keywords

  • DNA binding protein
  • protein structure
  • protein-DNA complex
  • synchrotron radiation
  • x-ray crystallography

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Structure of the gene V protein of bacteriophage f1 determined by multiwavelength x-ray diffraction on the selenomethionyl protein. / Skinner, Matthew M.; Zhang, Hong; Leschnitzer, Dale H.; Guan, Yue; Bellamy, Henry; Sweet, Robert M.; Gray, Carla W.; Konings, Ruud N H; Wang, Andrew H J; Terwilliger, Thomas C.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 91, No. 6, 15.03.1994, p. 2071-2075.

Research output: Contribution to journalArticle

Skinner, MM, Zhang, H, Leschnitzer, DH, Guan, Y, Bellamy, H, Sweet, RM, Gray, CW, Konings, RNH, Wang, AHJ & Terwilliger, TC 1994, 'Structure of the gene V protein of bacteriophage f1 determined by multiwavelength x-ray diffraction on the selenomethionyl protein', Proceedings of the National Academy of Sciences of the United States of America, vol. 91, no. 6, pp. 2071-2075.
Skinner, Matthew M. ; Zhang, Hong ; Leschnitzer, Dale H. ; Guan, Yue ; Bellamy, Henry ; Sweet, Robert M. ; Gray, Carla W. ; Konings, Ruud N H ; Wang, Andrew H J ; Terwilliger, Thomas C. / Structure of the gene V protein of bacteriophage f1 determined by multiwavelength x-ray diffraction on the selenomethionyl protein. In: Proceedings of the National Academy of Sciences of the United States of America. 1994 ; Vol. 91, No. 6. pp. 2071-2075.
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abstract = "The crystal structure of the dimeric gene V protein of bacteriophage f1 was determined using multiwavelength anomalous diffraction on the selenomethionine-containing wild-type and isoleucine-47 → methionine mutant proteins with x-ray diffraction data phased to 2.5 {\AA} resolution. The structure of the wild-type protein has been refined to an R factor of 19.2{\%} using native data to 1.8 {\AA} resolution. The structure of the gene V protein was used to obtain a model for the protein portion of the gene V protein- single-stranded DNA complex.",
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AU - Skinner, Matthew M.

AU - Zhang, Hong

AU - Leschnitzer, Dale H.

AU - Guan, Yue

AU - Bellamy, Henry

AU - Sweet, Robert M.

AU - Gray, Carla W.

AU - Konings, Ruud N H

AU - Wang, Andrew H J

AU - Terwilliger, Thomas C.

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