Study of the release of cell wall degrading enzymes during adhesion of Chlamydomonas gametes

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Abstract

A quantitative assay for cell wall release in Chlamydomonas has been used to study the timing of release of cell wall degrading enzyme (lysin) during adhesion. Lysin activity, which shows a broad pH range and requires divalent cations, is released as a pulse within 1-2 min after mixing of mt- and mt+ gametes. Thereafter, there is no further lysin release. Gametes of both mating types release the activity during aggregation with isolated gametic flagella of the opposite mating type, although mt+ gametes appear to release more lysin activity than mt- gametes. Electrophoretic analysis of cell wall proteins before and after lysin degradation indicate that the major wall proteins are unchanged after wall breakdown.

Original languageEnglish (US)
Pages (from-to)109-119
Number of pages11
JournalExperimental Cell Research
Volume138
Issue number1
DOIs
StatePublished - Mar 1982

ASJC Scopus subject areas

  • Cell Biology

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