TY - JOUR
T1 - Study of the release of cell wall degrading enzymes during adhesion of Chlamydomonas gametes
AU - Snell, William J.
PY - 1982/3
Y1 - 1982/3
N2 - A quantitative assay for cell wall release in Chlamydomonas has been used to study the timing of release of cell wall degrading enzyme (lysin) during adhesion. Lysin activity, which shows a broad pH range and requires divalent cations, is released as a pulse within 1-2 min after mixing of mt- and mt+ gametes. Thereafter, there is no further lysin release. Gametes of both mating types release the activity during aggregation with isolated gametic flagella of the opposite mating type, although mt+ gametes appear to release more lysin activity than mt- gametes. Electrophoretic analysis of cell wall proteins before and after lysin degradation indicate that the major wall proteins are unchanged after wall breakdown.
AB - A quantitative assay for cell wall release in Chlamydomonas has been used to study the timing of release of cell wall degrading enzyme (lysin) during adhesion. Lysin activity, which shows a broad pH range and requires divalent cations, is released as a pulse within 1-2 min after mixing of mt- and mt+ gametes. Thereafter, there is no further lysin release. Gametes of both mating types release the activity during aggregation with isolated gametic flagella of the opposite mating type, although mt+ gametes appear to release more lysin activity than mt- gametes. Electrophoretic analysis of cell wall proteins before and after lysin degradation indicate that the major wall proteins are unchanged after wall breakdown.
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U2 - 10.1016/0014-4827(82)90096-9
DO - 10.1016/0014-4827(82)90096-9
M3 - Article
C2 - 6802661
AN - SCOPUS:0019944210
SN - 0014-4827
VL - 138
SP - 109
EP - 119
JO - Experimental Cell Research
JF - Experimental Cell Research
IS - 1
ER -