Suppression of TRPC3 leads to disappearance of store-operated channels and formation of a new type of store-independent channels in A431 cells

Elena Kaznacheyeva, Lyuba Glushankova, Vladislav Bugaj, Olga Zimina, Anton Skopin, Vadim Alexeenko, Leonidas Tsiokas, Ilya Bezprozvanny, Galina N. Mozhayeva

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

In most non-excitable cells, calcium (Ca2+) release from the inositol 1,4,5-trisphosphate (InsP3)-sensitive intracellular Ca 2+ stores is coupled to Ca2+ influx through the plasma membrane Ca2+ channels whose molecular composition is poorly understood. Several members of mammalian TRP-related protein family have been implicated to both receptor- and store-operated Ca2+ influx. Here we investigated the role of the native transient receptor potential 3 (TRPC3) homologue in mediating the store- and receptor-operated calcium entry in A431 cells. We show that suppression of TRPC3 protein levels by small interfering RNA (siRNA) leads to a significant reduction in store-operated calcium influx without affecting the receptor-operated calcium influx. With single-channel analysis, we further demonstrate that reduction of TRPC3 levels results in suppression of specific subtype of store-operated calcium channels and activation of store-independent channels. Our data suggest that TRPC3 is required for the formation of functional store-operated channels in A431 cells.

Original languageEnglish (US)
Pages (from-to)23655-23662
Number of pages8
JournalJournal of Biological Chemistry
Volume282
Issue number32
DOIs
StatePublished - Aug 10 2007

Fingerprint

Calcium-Sensing Receptors
Calcium
Inositol 1,4,5-Trisphosphate
Calcium Channels
Cell membranes
Small Interfering RNA
Proteins
Chemical activation
Ion Channels
Chemical analysis
Cell Membrane

ASJC Scopus subject areas

  • Biochemistry

Cite this

Suppression of TRPC3 leads to disappearance of store-operated channels and formation of a new type of store-independent channels in A431 cells. / Kaznacheyeva, Elena; Glushankova, Lyuba; Bugaj, Vladislav; Zimina, Olga; Skopin, Anton; Alexeenko, Vadim; Tsiokas, Leonidas; Bezprozvanny, Ilya; Mozhayeva, Galina N.

In: Journal of Biological Chemistry, Vol. 282, No. 32, 10.08.2007, p. 23655-23662.

Research output: Contribution to journalArticle

Kaznacheyeva, E, Glushankova, L, Bugaj, V, Zimina, O, Skopin, A, Alexeenko, V, Tsiokas, L, Bezprozvanny, I & Mozhayeva, GN 2007, 'Suppression of TRPC3 leads to disappearance of store-operated channels and formation of a new type of store-independent channels in A431 cells', Journal of Biological Chemistry, vol. 282, no. 32, pp. 23655-23662. https://doi.org/10.1074/jbc.M608378200
Kaznacheyeva, Elena ; Glushankova, Lyuba ; Bugaj, Vladislav ; Zimina, Olga ; Skopin, Anton ; Alexeenko, Vadim ; Tsiokas, Leonidas ; Bezprozvanny, Ilya ; Mozhayeva, Galina N. / Suppression of TRPC3 leads to disappearance of store-operated channels and formation of a new type of store-independent channels in A431 cells. In: Journal of Biological Chemistry. 2007 ; Vol. 282, No. 32. pp. 23655-23662.
@article{2c0d13347b6d4169ac06885b4ec9168c,
title = "Suppression of TRPC3 leads to disappearance of store-operated channels and formation of a new type of store-independent channels in A431 cells",
abstract = "In most non-excitable cells, calcium (Ca2+) release from the inositol 1,4,5-trisphosphate (InsP3)-sensitive intracellular Ca 2+ stores is coupled to Ca2+ influx through the plasma membrane Ca2+ channels whose molecular composition is poorly understood. Several members of mammalian TRP-related protein family have been implicated to both receptor- and store-operated Ca2+ influx. Here we investigated the role of the native transient receptor potential 3 (TRPC3) homologue in mediating the store- and receptor-operated calcium entry in A431 cells. We show that suppression of TRPC3 protein levels by small interfering RNA (siRNA) leads to a significant reduction in store-operated calcium influx without affecting the receptor-operated calcium influx. With single-channel analysis, we further demonstrate that reduction of TRPC3 levels results in suppression of specific subtype of store-operated calcium channels and activation of store-independent channels. Our data suggest that TRPC3 is required for the formation of functional store-operated channels in A431 cells.",
author = "Elena Kaznacheyeva and Lyuba Glushankova and Vladislav Bugaj and Olga Zimina and Anton Skopin and Vadim Alexeenko and Leonidas Tsiokas and Ilya Bezprozvanny and Mozhayeva, {Galina N.}",
year = "2007",
month = "8",
day = "10",
doi = "10.1074/jbc.M608378200",
language = "English (US)",
volume = "282",
pages = "23655--23662",
journal = "Journal of Biological Chemistry",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology Inc.",
number = "32",

}

TY - JOUR

T1 - Suppression of TRPC3 leads to disappearance of store-operated channels and formation of a new type of store-independent channels in A431 cells

AU - Kaznacheyeva, Elena

AU - Glushankova, Lyuba

AU - Bugaj, Vladislav

AU - Zimina, Olga

AU - Skopin, Anton

AU - Alexeenko, Vadim

AU - Tsiokas, Leonidas

AU - Bezprozvanny, Ilya

AU - Mozhayeva, Galina N.

PY - 2007/8/10

Y1 - 2007/8/10

N2 - In most non-excitable cells, calcium (Ca2+) release from the inositol 1,4,5-trisphosphate (InsP3)-sensitive intracellular Ca 2+ stores is coupled to Ca2+ influx through the plasma membrane Ca2+ channels whose molecular composition is poorly understood. Several members of mammalian TRP-related protein family have been implicated to both receptor- and store-operated Ca2+ influx. Here we investigated the role of the native transient receptor potential 3 (TRPC3) homologue in mediating the store- and receptor-operated calcium entry in A431 cells. We show that suppression of TRPC3 protein levels by small interfering RNA (siRNA) leads to a significant reduction in store-operated calcium influx without affecting the receptor-operated calcium influx. With single-channel analysis, we further demonstrate that reduction of TRPC3 levels results in suppression of specific subtype of store-operated calcium channels and activation of store-independent channels. Our data suggest that TRPC3 is required for the formation of functional store-operated channels in A431 cells.

AB - In most non-excitable cells, calcium (Ca2+) release from the inositol 1,4,5-trisphosphate (InsP3)-sensitive intracellular Ca 2+ stores is coupled to Ca2+ influx through the plasma membrane Ca2+ channels whose molecular composition is poorly understood. Several members of mammalian TRP-related protein family have been implicated to both receptor- and store-operated Ca2+ influx. Here we investigated the role of the native transient receptor potential 3 (TRPC3) homologue in mediating the store- and receptor-operated calcium entry in A431 cells. We show that suppression of TRPC3 protein levels by small interfering RNA (siRNA) leads to a significant reduction in store-operated calcium influx without affecting the receptor-operated calcium influx. With single-channel analysis, we further demonstrate that reduction of TRPC3 levels results in suppression of specific subtype of store-operated calcium channels and activation of store-independent channels. Our data suggest that TRPC3 is required for the formation of functional store-operated channels in A431 cells.

UR - http://www.scopus.com/inward/record.url?scp=34548173034&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34548173034&partnerID=8YFLogxK

U2 - 10.1074/jbc.M608378200

DO - 10.1074/jbc.M608378200

M3 - Article

C2 - 17569672

AN - SCOPUS:34548173034

VL - 282

SP - 23655

EP - 23662

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

SN - 0021-9258

IS - 32

ER -