Suppression of TRPC3 leads to disappearance of store-operated channels and formation of a new type of store-independent channels in A431 cells

Elena Kaznacheyeva, Lyuba Glushankova, Vladislav Bugaj, Olga Zimina, Anton Skopin, Vadim Alexeenko, Leonidas Tsiokas, Ilya Bezprozvanny, Galina N. Mozhayeva

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

In most non-excitable cells, calcium (Ca2+) release from the inositol 1,4,5-trisphosphate (InsP3)-sensitive intracellular Ca 2+ stores is coupled to Ca2+ influx through the plasma membrane Ca2+ channels whose molecular composition is poorly understood. Several members of mammalian TRP-related protein family have been implicated to both receptor- and store-operated Ca2+ influx. Here we investigated the role of the native transient receptor potential 3 (TRPC3) homologue in mediating the store- and receptor-operated calcium entry in A431 cells. We show that suppression of TRPC3 protein levels by small interfering RNA (siRNA) leads to a significant reduction in store-operated calcium influx without affecting the receptor-operated calcium influx. With single-channel analysis, we further demonstrate that reduction of TRPC3 levels results in suppression of specific subtype of store-operated calcium channels and activation of store-independent channels. Our data suggest that TRPC3 is required for the formation of functional store-operated channels in A431 cells.

Original languageEnglish (US)
Pages (from-to)23655-23662
Number of pages8
JournalJournal of Biological Chemistry
Volume282
Issue number32
DOIs
StatePublished - Aug 10 2007

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ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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