Susceptibility of Human Embryonic Stem Cell-Derived Neural Cells to Japanese Encephalitis Virus Infection

Shih Cheng Shen, Ching I. Shen, Ho Lin, Chun Jung Chen, Chia Yu Chang, Sheng Mei Chen, Hsiu Chin Lee, Ping Shan Lai, Hong Lin Su

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Pluripotent human embryonic stem cells (hESCs) can be efficiently directed to become immature neuroepithelial precursor cells (NPCs) and functional mature neural cells, including neurotransmitter-secreting neurons and glial cells. Investigating the susceptibility of these hESCs-derived neural cells to neurotrophic viruses, such as Japanese encephalitis virus (JEV), provides insight into the viral cell tropism in the infected human brain. We demonstrate that hESC-derived NPCs are highly vulnerable to JEV infection at a low multiplicity of infection (MOI). In addition, glial fibrillary acid protein (GFAP)-expressing glial cells are also susceptible to JEV infection. In contrast, only a few mature neurons were infected at MOI 10 or higher on the third day post-infection. In addition, functional neurotransmitter-secreting neurons are also resistant to JEV infection at high MOI. Moreover, we discover that vimentin intermediate filament, reported as a putative neurovirulent JEV receptor, is highly expressed in NPCs and glial cells, but not mature neurons. These results indicate that the expression of vimentin in neural cells correlates to the cell tropism of JEV. Finally, we further demonstrate that membranous vimentin is necessary for the susceptibility of hESC-derived NPCs to JEV infection.

Original languageEnglish (US)
Article numbere114990
JournalPLoS One
Volume9
Issue number12
DOIs
StatePublished - Dec 17 2014

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Japanese Encephalitis Virus
Japanese encephalitis virus
embryonic stem cells
Stem cells
Viruses
neurons
Neuroepithelial Cells
neuroglia
Neurons
Infection
infection
Vimentin
vimentin
Neuroglia
tropisms
cells
neurotransmitters
Neurotransmitter Agents
Viral Tropism
Virus Receptors

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Shen, S. C., Shen, C. I., Lin, H., Chen, C. J., Chang, C. Y., Chen, S. M., ... Su, H. L. (2014). Susceptibility of Human Embryonic Stem Cell-Derived Neural Cells to Japanese Encephalitis Virus Infection. PLoS One, 9(12), [e114990]. https://doi.org/10.1371/journal.pone.0114990

Susceptibility of Human Embryonic Stem Cell-Derived Neural Cells to Japanese Encephalitis Virus Infection. / Shen, Shih Cheng; Shen, Ching I.; Lin, Ho; Chen, Chun Jung; Chang, Chia Yu; Chen, Sheng Mei; Lee, Hsiu Chin; Lai, Ping Shan; Su, Hong Lin.

In: PLoS One, Vol. 9, No. 12, e114990, 17.12.2014.

Research output: Contribution to journalArticle

Shen, SC, Shen, CI, Lin, H, Chen, CJ, Chang, CY, Chen, SM, Lee, HC, Lai, PS & Su, HL 2014, 'Susceptibility of Human Embryonic Stem Cell-Derived Neural Cells to Japanese Encephalitis Virus Infection', PLoS One, vol. 9, no. 12, e114990. https://doi.org/10.1371/journal.pone.0114990
Shen, Shih Cheng ; Shen, Ching I. ; Lin, Ho ; Chen, Chun Jung ; Chang, Chia Yu ; Chen, Sheng Mei ; Lee, Hsiu Chin ; Lai, Ping Shan ; Su, Hong Lin. / Susceptibility of Human Embryonic Stem Cell-Derived Neural Cells to Japanese Encephalitis Virus Infection. In: PLoS One. 2014 ; Vol. 9, No. 12.
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