Synaptophysin, a major synaptic vesicle protein, is not essential for neurotransmitter release

Harvey T. Mcmahon, Vadim Y. Bolshakov, Roger Janz, Robert E Hammer, Steven A. Siegelbaum, Thomas C. Südhof

Research output: Contribution to journalArticle

185 Citations (Scopus)

Abstract

Synaptophysin (syp I) is a synaptic vesicle membrane protein that constitutes ≃7% of the total vesicle protein. Multiple lines of evidence implicate syp I in a number of nerve terminal functions. To test these, we have disrupted the murine syp I gene. Mutant mice lacking syp I were viable and fertile. No changes in the structure and protein composition of the mutant brains were observed except for a decrease in synaptobrevin/VAMP II. Synaptic transmission was normal with no detectable changes in synaptic plasticity or the probability of release. Our data demonstrate that one of the major synaptic vesicle membrane proteins is not essential for synaptic transmission, suggesting that its function is either redundant or that it has a more subtle function not apparent in the assays used.

Original languageEnglish (US)
Pages (from-to)4760-4764
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume93
Issue number10
DOIs
StatePublished - May 14 1996

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Synaptic Membranes
Synaptophysin
Synaptic Vesicles
Synaptic Transmission
Neurotransmitter Agents
Membrane Proteins
R-SNARE Proteins
Neuronal Plasticity
Mutant Proteins
Proteins
Brain
Genes

ASJC Scopus subject areas

  • Genetics
  • General

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Synaptophysin, a major synaptic vesicle protein, is not essential for neurotransmitter release. / Mcmahon, Harvey T.; Bolshakov, Vadim Y.; Janz, Roger; Hammer, Robert E; Siegelbaum, Steven A.; Südhof, Thomas C.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, No. 10, 14.05.1996, p. 4760-4764.

Research output: Contribution to journalArticle

Mcmahon, Harvey T. ; Bolshakov, Vadim Y. ; Janz, Roger ; Hammer, Robert E ; Siegelbaum, Steven A. ; Südhof, Thomas C. / Synaptophysin, a major synaptic vesicle protein, is not essential for neurotransmitter release. In: Proceedings of the National Academy of Sciences of the United States of America. 1996 ; Vol. 93, No. 10. pp. 4760-4764.
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