Syndet is a novel SNAP-25 related protein expressed in many tissues

Guangyi Wang, Joan W. Witkin, Gianming Hao, Vytas A. Bankaitis, Philipp E. Scherer, Giulia Baldini

Research output: Contribution to journalArticle

88 Citations (Scopus)

Abstract

SNAP-25 is a synaptosomal associated protein localized at the plasma membrane of nerve terminals. SNAP-25 associates with syntaxin 1 and vesicle-associated membrane protein-2 (VAMP-2) and is thought to form a complex essential for neurotransmitter release. We have identified syndet, a novel protein related to the family of SNAP-25 isoforms. Like SNAP-25, syndet has regions with high probability of forming coiled coils, a cysteine rich-domain, and lacks a signal sequence or transmembrane domains. Syndet is tightly bound to membranes, possibly by acylation within the cysteine-rich domain. Syndet is expressed in non-neuronal tissues. In adipocytes, syndet is found at the plasma membrane and in an intracellular compartment. The identification of syndet supports the hypothesis that multiple SNAP-25 related proteins ensure specificity of vesicle fusion at the cell surface.

Original languageEnglish (US)
Pages (from-to)505-513
Number of pages9
JournalJournal of Cell Science
Volume110
Issue number4
StatePublished - Feb 1997

Fingerprint

Synaptosomal-Associated Protein 25
Detergents
Cysteine
Vesicle-Associated Membrane Protein 2
Syntaxin 1
Cell Membrane
Acylation
Cell Fusion
Protein Sorting Signals
Adipocytes
Neurotransmitter Agents
Protein Isoforms
Proteins
Membranes

Keywords

  • SNAP-25
  • Syndet
  • t-SNARE

ASJC Scopus subject areas

  • Cell Biology

Cite this

Wang, G., Witkin, J. W., Hao, G., Bankaitis, V. A., Scherer, P. E., & Baldini, G. (1997). Syndet is a novel SNAP-25 related protein expressed in many tissues. Journal of Cell Science, 110(4), 505-513.

Syndet is a novel SNAP-25 related protein expressed in many tissues. / Wang, Guangyi; Witkin, Joan W.; Hao, Gianming; Bankaitis, Vytas A.; Scherer, Philipp E.; Baldini, Giulia.

In: Journal of Cell Science, Vol. 110, No. 4, 02.1997, p. 505-513.

Research output: Contribution to journalArticle

Wang, G, Witkin, JW, Hao, G, Bankaitis, VA, Scherer, PE & Baldini, G 1997, 'Syndet is a novel SNAP-25 related protein expressed in many tissues', Journal of Cell Science, vol. 110, no. 4, pp. 505-513.
Wang G, Witkin JW, Hao G, Bankaitis VA, Scherer PE, Baldini G. Syndet is a novel SNAP-25 related protein expressed in many tissues. Journal of Cell Science. 1997 Feb;110(4):505-513.
Wang, Guangyi ; Witkin, Joan W. ; Hao, Gianming ; Bankaitis, Vytas A. ; Scherer, Philipp E. ; Baldini, Giulia. / Syndet is a novel SNAP-25 related protein expressed in many tissues. In: Journal of Cell Science. 1997 ; Vol. 110, No. 4. pp. 505-513.
@article{75ffe40336d24718afd9b816d7c32edd,
title = "Syndet is a novel SNAP-25 related protein expressed in many tissues",
abstract = "SNAP-25 is a synaptosomal associated protein localized at the plasma membrane of nerve terminals. SNAP-25 associates with syntaxin 1 and vesicle-associated membrane protein-2 (VAMP-2) and is thought to form a complex essential for neurotransmitter release. We have identified syndet, a novel protein related to the family of SNAP-25 isoforms. Like SNAP-25, syndet has regions with high probability of forming coiled coils, a cysteine rich-domain, and lacks a signal sequence or transmembrane domains. Syndet is tightly bound to membranes, possibly by acylation within the cysteine-rich domain. Syndet is expressed in non-neuronal tissues. In adipocytes, syndet is found at the plasma membrane and in an intracellular compartment. The identification of syndet supports the hypothesis that multiple SNAP-25 related proteins ensure specificity of vesicle fusion at the cell surface.",
keywords = "SNAP-25, Syndet, t-SNARE",
author = "Guangyi Wang and Witkin, {Joan W.} and Gianming Hao and Bankaitis, {Vytas A.} and Scherer, {Philipp E.} and Giulia Baldini",
year = "1997",
month = "2",
language = "English (US)",
volume = "110",
pages = "505--513",
journal = "Journal of Cell Science",
issn = "0021-9533",
publisher = "Company of Biologists Ltd",
number = "4",

}

TY - JOUR

T1 - Syndet is a novel SNAP-25 related protein expressed in many tissues

AU - Wang, Guangyi

AU - Witkin, Joan W.

AU - Hao, Gianming

AU - Bankaitis, Vytas A.

AU - Scherer, Philipp E.

AU - Baldini, Giulia

PY - 1997/2

Y1 - 1997/2

N2 - SNAP-25 is a synaptosomal associated protein localized at the plasma membrane of nerve terminals. SNAP-25 associates with syntaxin 1 and vesicle-associated membrane protein-2 (VAMP-2) and is thought to form a complex essential for neurotransmitter release. We have identified syndet, a novel protein related to the family of SNAP-25 isoforms. Like SNAP-25, syndet has regions with high probability of forming coiled coils, a cysteine rich-domain, and lacks a signal sequence or transmembrane domains. Syndet is tightly bound to membranes, possibly by acylation within the cysteine-rich domain. Syndet is expressed in non-neuronal tissues. In adipocytes, syndet is found at the plasma membrane and in an intracellular compartment. The identification of syndet supports the hypothesis that multiple SNAP-25 related proteins ensure specificity of vesicle fusion at the cell surface.

AB - SNAP-25 is a synaptosomal associated protein localized at the plasma membrane of nerve terminals. SNAP-25 associates with syntaxin 1 and vesicle-associated membrane protein-2 (VAMP-2) and is thought to form a complex essential for neurotransmitter release. We have identified syndet, a novel protein related to the family of SNAP-25 isoforms. Like SNAP-25, syndet has regions with high probability of forming coiled coils, a cysteine rich-domain, and lacks a signal sequence or transmembrane domains. Syndet is tightly bound to membranes, possibly by acylation within the cysteine-rich domain. Syndet is expressed in non-neuronal tissues. In adipocytes, syndet is found at the plasma membrane and in an intracellular compartment. The identification of syndet supports the hypothesis that multiple SNAP-25 related proteins ensure specificity of vesicle fusion at the cell surface.

KW - SNAP-25

KW - Syndet

KW - t-SNARE

UR - http://www.scopus.com/inward/record.url?scp=0031049776&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031049776&partnerID=8YFLogxK

M3 - Article

VL - 110

SP - 505

EP - 513

JO - Journal of Cell Science

JF - Journal of Cell Science

SN - 0021-9533

IS - 4

ER -