Synthesis and biological activity of glycosylated analogs of somatostatin

S. Lavielle, N. Ling, P. Brazeau, R. Benoit, T. Wasada, D. Harris, Roger H Unger, R. Guillemin

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The synthesis by solid-phase methodology of two glycosylated analogs of somatostatin [Glc-Asn5]-SS and [NAcGlc-Asn5]-SS is described. These two analogs have been biologically tested on the secretion of pituitary growth hormone, pancreatic glucagon and insulin. The results show that glycosylation of somatostatin on the Asn5 residue decreases by a hundred fold the inhibition activity on GH release when tested invitro. Invivo, since the activity is similar to somatostatin the carbohydrates are probably removed by some enzymatic reaction and thus liberate the full activity of somatostatin.

Original languageEnglish (US)
Pages (from-to)614-622
Number of pages9
JournalBiochemical and Biophysical Research Communications
Volume91
Issue number2
DOIs
StatePublished - Nov 28 1979

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Bioactivity
Somatostatin
Glycosylation
Solid-Phase Synthesis Techniques
Glucagon
Growth Hormone
Carbohydrates
Insulin

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Lavielle, S., Ling, N., Brazeau, P., Benoit, R., Wasada, T., Harris, D., ... Guillemin, R. (1979). Synthesis and biological activity of glycosylated analogs of somatostatin. Biochemical and Biophysical Research Communications, 91(2), 614-622. https://doi.org/10.1016/0006-291X(79)91566-3

Synthesis and biological activity of glycosylated analogs of somatostatin. / Lavielle, S.; Ling, N.; Brazeau, P.; Benoit, R.; Wasada, T.; Harris, D.; Unger, Roger H; Guillemin, R.

In: Biochemical and Biophysical Research Communications, Vol. 91, No. 2, 28.11.1979, p. 614-622.

Research output: Contribution to journalArticle

Lavielle, S, Ling, N, Brazeau, P, Benoit, R, Wasada, T, Harris, D, Unger, RH & Guillemin, R 1979, 'Synthesis and biological activity of glycosylated analogs of somatostatin', Biochemical and Biophysical Research Communications, vol. 91, no. 2, pp. 614-622. https://doi.org/10.1016/0006-291X(79)91566-3
Lavielle, S. ; Ling, N. ; Brazeau, P. ; Benoit, R. ; Wasada, T. ; Harris, D. ; Unger, Roger H ; Guillemin, R. / Synthesis and biological activity of glycosylated analogs of somatostatin. In: Biochemical and Biophysical Research Communications. 1979 ; Vol. 91, No. 2. pp. 614-622.
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