Synucleins in glaucoma: Implication of γ-synuclein in glaucomatous alterations in the optic nerve

Irina Surgucheva, Belinda McMahan, Farid Ahmed, Stanislav Tomarev, Martin B. Wax, Andrei Surguchov

Research output: Contribution to journalArticle

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Abstract

Synucleins are small proteins associated with neurodegenerative diseases and some forms of cancer. They are studied predominantly in the brain; information about their presence and functions in ocular tissues is scarce. Here we describe the localization of three members of the synuclein family in the optic nerve of donors with different types of glaucoma compared with control samples from donors without ocular diseases. We did not find significant differences in the localization of α- and β-synucleins in the optic nerve or retina of glaucoma patients compared with controls, whereas considerable redistribution of γ-synuclein occurred in the glaucomatous optic nerve compared with control eye without glaucoma. In the optic nerve from control and glaucomatous individuals, nerve bundles are immunopositive for γ-synuclein; however, a strong γ-synuclein-immunopositive staining in a subset of glial cells was observed in the lamina and postlamina cribrosa regions of the optic nerve only in glaucoma patients. In the optic nerve of rats with episcleral vein cauterization used as an animal model of glaucoma, the quantity of both γ-synuclein mRNA and protein was decreased compared with the optic nerves of control animals. Incubation of rat astrocyte culture at elevated hydrostatic pressure reduced the amount of γ-synuclein but did not affect the quantities of actin and glial fibrillary acidic protein. These data suggest that significant changes in the pattern of expression and/or localization occur in the glaucomatous optic nerve for γ-synuclein but not for α- and β-members of the synuclein family.

Original languageEnglish (US)
Pages (from-to)97-106
Number of pages10
JournalJournal of Neuroscience Research
Volume68
Issue number1
DOIs
StatePublished - 2002

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Synucleins
Optic Nerve
Glaucoma
Tissue Donors
Cautery
Hydrostatic Pressure
Eye Diseases
Glial Fibrillary Acidic Protein
Neuroglia
Astrocytes
Neurodegenerative Diseases
Retina
Actins
Veins
Proteins
Animal Models

Keywords

  • γ-synuclein
  • Astrocytes
  • Cell models
  • Centrosomes
  • Nucleus
  • Optic nerve

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Synucleins in glaucoma : Implication of γ-synuclein in glaucomatous alterations in the optic nerve. / Surgucheva, Irina; McMahan, Belinda; Ahmed, Farid; Tomarev, Stanislav; Wax, Martin B.; Surguchov, Andrei.

In: Journal of Neuroscience Research, Vol. 68, No. 1, 2002, p. 97-106.

Research output: Contribution to journalArticle

Surgucheva, I, McMahan, B, Ahmed, F, Tomarev, S, Wax, MB & Surguchov, A 2002, 'Synucleins in glaucoma: Implication of γ-synuclein in glaucomatous alterations in the optic nerve', Journal of Neuroscience Research, vol. 68, no. 1, pp. 97-106. https://doi.org/10.1002/jnr.10198
Surgucheva, Irina ; McMahan, Belinda ; Ahmed, Farid ; Tomarev, Stanislav ; Wax, Martin B. ; Surguchov, Andrei. / Synucleins in glaucoma : Implication of γ-synuclein in glaucomatous alterations in the optic nerve. In: Journal of Neuroscience Research. 2002 ; Vol. 68, No. 1. pp. 97-106.
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