T-STAR/ETOILE: A novel relative of SAM68 that interacts with an RNA-binding protein implicated in spermatogenesis

J. P. Venables, C. Vernet, S. L. Chew, D. J. Elliott, R. B. Cowmeadow, J. Wu, H. J. Cooke, K. Artzt, I. C. Eperon

Research output: Contribution to journalArticlepeer-review

102 Scopus citations


RBM is an RNA-binding protein encoded on the Y chromosome in mammals and is expressed only in the nuclei of male germ cells. Genetic evidence from infertile men implicates it in spermatogenesis, but its function is unknown. Of a number of potential partners for RBM identified by a yeast two-hybrid screen with testis cDNA, the most frequent isolates encoded a novel RNA-binding protein, termed T-STAR, that is closely related to SAM68, an Src-associated protein of unknown function. The mouse homologue was also cloned and designated etoile. It mapped to chromosome 15, while T-STAR mapped to the syntenic region on human chromosome 8. T-STAR/etoile is expressed primarily in the testis; in rat germ cells, the expression of both T-STAR/etoile and SAM68 is regulated during meiosis. Transfection of T-STAR/etoile fused with green fluorescent protein into HeLa cells caused an accumulation of protein in a novel compartment of the nucleus, adjacent to the nucleolus but distinct from the peri-nucleolar compartment. RBM and other hnRNP G family members are candidate downstream targets for regulation by T-STAR/ETOILE and SAM68.

Original languageEnglish (US)
Pages (from-to)959-969
Number of pages11
JournalHuman molecular genetics
Issue number6
StatePublished - 1999

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Genetics(clinical)


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