T3SS effector VopL inhibits the host ROS response, promoting the intracellular survival of Vibrio parahaemolyticus

Marcela de Souza Santos, Dor Salomon, Kim Orth

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The production of antimicrobial reactive oxygen species by the nicotinamide dinucleotide phosphate (NADPH) oxidase complex is an important mechanism for control of invading pathogens. Herein, we show that the gastrointestinal pathogen Vibrio parahaemolyticus counteracts reactive oxygen species (ROS) production using the Type III Secretion System 2 (T3SS2) effector VopL. In the absence of VopL, intracellular V. parahaemolyticus undergoes ROS-dependent filamentation, with concurrent limited growth. During infection, VopL assembles actin into non-functional filaments resulting in a dysfunctional actin cytoskeleton that can no longer mediate the assembly of the NADPH oxidase at the cell membrane, thereby limiting ROS production. This is the first example of how a T3SS2 effector contributes to the intracellular survival of V. parahaemolyticus, supporting the establishment of a protective intracellular replicative niche.

Original languageEnglish (US)
Article numbere1006438
JournalPLoS Pathogens
Volume13
Issue number6
DOIs
StatePublished - 2017

Fingerprint

Vibrio parahaemolyticus
Reactive Oxygen Species
Niacinamide
Oxidoreductases
Phosphates
Actin Cytoskeleton
Actins
Cell Membrane
Growth
Infection

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Molecular Biology
  • Genetics
  • Virology

Cite this

T3SS effector VopL inhibits the host ROS response, promoting the intracellular survival of Vibrio parahaemolyticus. / de Souza Santos, Marcela; Salomon, Dor; Orth, Kim.

In: PLoS Pathogens, Vol. 13, No. 6, e1006438, 2017.

Research output: Contribution to journalArticle

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