Targeted therapy for the loss of von Hippel-Lindau in renal cell carcinoma: A novel molecule that induces autophagic cell death

Sandra Turcotte, Patrick D. Sutphin, Amato J. Giaccia

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

Radiation and conventional cytotoxic chemotherapies are ineffective in treating renal cancer. Approximately 75 percent of renal cell carcinoma (RCC) is associated with an inactivation of the tumor suppressor gene von Hippel-Lindau (VHL). We exploited the possibility of targeting VHL-deficient RCC through synthetic lethality using a high-throughput screening approach. In this screen, STF-62247 was identified to be selectively toxic and growth inhibitory to renal cells lacking VHL. We recendy demonstrated that the cytotoxicity of STF-62247 is due to dysregulated autophagy. Furthermore, the reduction of protein levels of essential autophagy pathway components such as Atg5, Atg7 and Atg9 reduces sensitivity of VHL-deficient cells to killing by STF-62247. Loss of proteins involved in Golgi trafficking sensitized RCC with wild-type VHL to killing by STF-62247, indicating a potential role for these proteins as a target of the compound. Our study supports the concept of using synthetic lethality to selectively kill VHL-deficient cells that represents a new type of targeted therapy for the treatment of RCC.

Original languageEnglish (US)
Pages (from-to)944-946
Number of pages3
JournalAutophagy
Volume4
Issue number7
Publication statusPublished - Oct 1 2008

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Keywords

  • Autophagosomes
  • Autophagy
  • Cell death
  • Kidney cancer
  • LC3
  • Renal cell carcinoma
  • Targeted therapy
  • Von Hippel-Lindau

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

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