Telomere biology and cellular aging in nonhuman primate cells

Susanne Steinert, Dennis M. White, Ying Zou, Jerry W. Shay, Woodring E. Wright

Research output: Contribution to journalArticle

81 Citations (Scopus)

Abstract

To determine how cellular aging is conserved among primates, we analyzed the replicative potential and telomere shortening in skin fibroblasts of anthropoids and prosimians. The average telomere length of the New World primates Ateles geoffroyi (spider monkey) and Saimiri sciureus (squirrel monkey) and the Old World primates Macaca mulatta (rhesus monkey), Pongo pygmaeus (orangutan), and Pan paniscus (pigmy chimpanzee) ranged from 4 to 16 kb. We found that telomere shortening limits the replicative capacity of anthropoid fibroblasts and that the expression of human telomerase produced telomere elongation and the extension of their in vitro life span. In contrast the prosimian Lemur catta (ring-tailed lemur) had both long and short telomeres and telomere shortening did not provide an absolute barrier to immortalization. Following a transient growth arrest a subset of cells showing a reduced number of chromosomes overgrew the cultures without activation of telomerase. Here we show that the presence of continuous TTAGGG repeats at telomeres and rigorous control of replicative aging by telomere shortening appear to be conserved among anthropoid primates but is less effective in prosimian lemurs.

Original languageEnglish (US)
Pages (from-to)146-152
Number of pages7
JournalExperimental Cell Research
Volume272
Issue number2
DOIs
StatePublished - 2002

Fingerprint

Telomere Shortening
Cell Aging
Lemur
Strepsirhini
Telomere
Primates
Atelinae
Haplorhini
Saimiri
Telomerase
Macaca mulatta
Pongo pygmaeus
Pan paniscus
Fibroblasts
Pongo
Pan troglodytes
Chromosomes
Skin
Growth

Keywords

  • Anthropoids
  • Primates
  • Prosimians
  • Replicative aging
  • Telomerase
  • Telomeres

ASJC Scopus subject areas

  • Cell Biology

Cite this

Telomere biology and cellular aging in nonhuman primate cells. / Steinert, Susanne; White, Dennis M.; Zou, Ying; Shay, Jerry W.; Wright, Woodring E.

In: Experimental Cell Research, Vol. 272, No. 2, 2002, p. 146-152.

Research output: Contribution to journalArticle

Steinert, Susanne ; White, Dennis M. ; Zou, Ying ; Shay, Jerry W. ; Wright, Woodring E. / Telomere biology and cellular aging in nonhuman primate cells. In: Experimental Cell Research. 2002 ; Vol. 272, No. 2. pp. 146-152.
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