Telomere G-overhang length measurement method 1: the DSN method.

Yong Zhao, Jerry W. Shay, Woodring E. Wright

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Telomeres terminate in 3' single-stranded G-overhangs that function in telomere end protection and telomerase action. An accurate measurement of overhang length is challenging due to the presence of many kilobases of double-stranded telomere DNA. Here, a simple method is described that utilizes duplex-specific nuclease (DSN) to digest all genomic DNA including telomeres, leaving the single-stranded overhangs intact. The telomere single-strand G-rich overhang length can then be determined by Southern blot-based assays.

Original languageEnglish (US)
Pages (from-to)47-54
Number of pages8
JournalMethods in molecular biology (Clifton, N.J.)
Volume735
DOIs
StatePublished - Aug 5 2011

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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