Telomere G-overhang length measurement method 1: the DSN method.

Yong Zhao; Jerry W. Shay; Woodring E. Wright

doi:10.1007/978-1-61779-092-8_5

Telomere G-overhang length measurement method 1: the DSN method.

Yong Zhao, Jerry W. Shay, Woodring E. Wright

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

Telomeres terminate in 3' single-stranded G-overhangs that function in telomere end protection and telomerase action. An accurate measurement of overhang length is challenging due to the presence of many kilobases of double-stranded telomere DNA. Here, a simple method is described that utilizes duplex-specific nuclease (DSN) to digest all genomic DNA including telomeres, leaving the single-stranded overhangs intact. The telomere single-strand G-rich overhang length can then be determined by Southern blot-based assays.

Original language	English (US)
Pages (from-to)	47-54
Number of pages	8
Journal	Methods in molecular biology (Clifton, N.J.)
Volume	735
DOIs	https://doi.org/10.1007/978-1-61779-092-8_5
State	Published - 2011

ASJC Scopus subject areas

Molecular Biology
Genetics

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10.1007/978-1-61779-092-8_5

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abstract = "Telomeres terminate in 3' single-stranded G-overhangs that function in telomere end protection and telomerase action. An accurate measurement of overhang length is challenging due to the presence of many kilobases of double-stranded telomere DNA. Here, a simple method is described that utilizes duplex-specific nuclease (DSN) to digest all genomic DNA including telomeres, leaving the single-stranded overhangs intact. The telomere single-strand G-rich overhang length can then be determined by Southern blot-based assays.",

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AU - Shay, Jerry W.

AU - Wright, Woodring E.

PY - 2011

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N2 - Telomeres terminate in 3' single-stranded G-overhangs that function in telomere end protection and telomerase action. An accurate measurement of overhang length is challenging due to the presence of many kilobases of double-stranded telomere DNA. Here, a simple method is described that utilizes duplex-specific nuclease (DSN) to digest all genomic DNA including telomeres, leaving the single-stranded overhangs intact. The telomere single-strand G-rich overhang length can then be determined by Southern blot-based assays.

AB - Telomeres terminate in 3' single-stranded G-overhangs that function in telomere end protection and telomerase action. An accurate measurement of overhang length is challenging due to the presence of many kilobases of double-stranded telomere DNA. Here, a simple method is described that utilizes duplex-specific nuclease (DSN) to digest all genomic DNA including telomeres, leaving the single-stranded overhangs intact. The telomere single-strand G-rich overhang length can then be determined by Southern blot-based assays.

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Telomere G-overhang length measurement method 1: the DSN method.

Abstract

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