Telomere G-overhang length measurement method 1: the DSN method.

Yong Zhao, Jerry W. Shay, Woodring E. Wright

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Telomeres terminate in 3' single-stranded G-overhangs that function in telomere end protection and telomerase action. An accurate measurement of overhang length is challenging due to the presence of many kilobases of double-stranded telomere DNA. Here, a simple method is described that utilizes duplex-specific nuclease (DSN) to digest all genomic DNA including telomeres, leaving the single-stranded overhangs intact. The telomere single-strand G-rich overhang length can then be determined by Southern blot-based assays.

Original languageEnglish (US)
Pages (from-to)47-54
Number of pages8
JournalMethods in molecular biology (Clifton, N.J.)
Volume735
DOIs
StatePublished - 2011

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Telomere
Telomerase
DNA
Southern Blotting

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Telomere G-overhang length measurement method 1 : the DSN method. / Zhao, Yong; Shay, Jerry W.; Wright, Woodring E.

In: Methods in molecular biology (Clifton, N.J.), Vol. 735, 2011, p. 47-54.

Research output: Contribution to journalArticle

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