Temporospatial expression of the small HSP/αB-crystallin in cardiac and skeletal muscle during mouse development

Ivor J. Benjamin, John Shelton, Daniel J. Garry, James A. Richardson

Research output: Contribution to journalArticle

71 Citations (Scopus)

Abstract

Although the small (22 Kd) heat shock protein/αB-crystallin functions as a major structural protein and molecular chaperone in the vertebrate lens, little is known about the protein's role in nonlenticular tissues such as the heart and skeletal muscle. Recent studies have demonstrated that αB- crystallin expression is uniquely regulated during myogenesis in vitro. We report here for the first time that the temporal and spatial expression of αB-crystallin is similarly regulated in vivo during mouse embryogenesis. Expression of αB-crystallin mRNA was detected by in situ hybridization in the primitive heart at 8.5 days postconception (p.c.) and in the myotome of the somites at 10.5 days p.c. This tissue-restricted pattern was corroborated by immunohistochemical studies. αB-crystallin mRNA and protein expression were uniform in the developing atria and ventricles without regional differences or gradients. αB-crystallin expression was absent in the endocardial cushion, pulmonary trunk, aorta, and endothelium. Examination of muscle precursors revealed expression throughout the dorsoventral aspect of the myotomes and in developing skeletal muscle. Our findings suggest that αB-crystallin may serve pivotal roles as a structural protein and a molecular chaperone in myofiber stabilization of metabolically active tissues during early embryogenesis. Thus, early αB-crystallin expression in myogenic lineages supports the hypothesis that the putative functions of αB- crystallin are coupled to the activation of genetic programs responsible for myogenic differentiation and cardiac morphogenesis.

Original languageEnglish (US)
Pages (from-to)75-84
Number of pages10
JournalDevelopmental Dynamics
Volume208
Issue number1
DOIs
StatePublished - Jan 1997

Fingerprint

Crystallins
Myocardium
Skeletal Muscle
Molecular Chaperones
Embryonic Development
Proteins
Endocardial Cushions
Somites
Messenger RNA
Muscle Development
Heat-Shock Proteins
Morphogenesis
Lenses
Endothelium
In Situ Hybridization
Aorta
Vertebrates
Muscles
Lung

Keywords

  • αB-crystallin
  • development
  • heart
  • immunohistochemistry
  • in situ hybridization
  • mouse
  • skeletal muscle

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

Cite this

Temporospatial expression of the small HSP/αB-crystallin in cardiac and skeletal muscle during mouse development. / Benjamin, Ivor J.; Shelton, John; Garry, Daniel J.; Richardson, James A.

In: Developmental Dynamics, Vol. 208, No. 1, 01.1997, p. 75-84.

Research output: Contribution to journalArticle

@article{1600de8d25844b14b7fecb0d6fc4c869,
title = "Temporospatial expression of the small HSP/αB-crystallin in cardiac and skeletal muscle during mouse development",
abstract = "Although the small (22 Kd) heat shock protein/αB-crystallin functions as a major structural protein and molecular chaperone in the vertebrate lens, little is known about the protein's role in nonlenticular tissues such as the heart and skeletal muscle. Recent studies have demonstrated that αB- crystallin expression is uniquely regulated during myogenesis in vitro. We report here for the first time that the temporal and spatial expression of αB-crystallin is similarly regulated in vivo during mouse embryogenesis. Expression of αB-crystallin mRNA was detected by in situ hybridization in the primitive heart at 8.5 days postconception (p.c.) and in the myotome of the somites at 10.5 days p.c. This tissue-restricted pattern was corroborated by immunohistochemical studies. αB-crystallin mRNA and protein expression were uniform in the developing atria and ventricles without regional differences or gradients. αB-crystallin expression was absent in the endocardial cushion, pulmonary trunk, aorta, and endothelium. Examination of muscle precursors revealed expression throughout the dorsoventral aspect of the myotomes and in developing skeletal muscle. Our findings suggest that αB-crystallin may serve pivotal roles as a structural protein and a molecular chaperone in myofiber stabilization of metabolically active tissues during early embryogenesis. Thus, early αB-crystallin expression in myogenic lineages supports the hypothesis that the putative functions of αB- crystallin are coupled to the activation of genetic programs responsible for myogenic differentiation and cardiac morphogenesis.",
keywords = "αB-crystallin, development, heart, immunohistochemistry, in situ hybridization, mouse, skeletal muscle",
author = "Benjamin, {Ivor J.} and John Shelton and Garry, {Daniel J.} and Richardson, {James A.}",
year = "1997",
month = "1",
doi = "10.1002/(SICI)1097-0177(199701)208:1<75::AID-AJA7>3.0.CO;2-Z",
language = "English (US)",
volume = "208",
pages = "75--84",
journal = "Developmental Dynamics",
issn = "1058-8388",
publisher = "Wiley-Liss Inc.",
number = "1",

}

TY - JOUR

T1 - Temporospatial expression of the small HSP/αB-crystallin in cardiac and skeletal muscle during mouse development

AU - Benjamin, Ivor J.

AU - Shelton, John

AU - Garry, Daniel J.

AU - Richardson, James A.

PY - 1997/1

Y1 - 1997/1

N2 - Although the small (22 Kd) heat shock protein/αB-crystallin functions as a major structural protein and molecular chaperone in the vertebrate lens, little is known about the protein's role in nonlenticular tissues such as the heart and skeletal muscle. Recent studies have demonstrated that αB- crystallin expression is uniquely regulated during myogenesis in vitro. We report here for the first time that the temporal and spatial expression of αB-crystallin is similarly regulated in vivo during mouse embryogenesis. Expression of αB-crystallin mRNA was detected by in situ hybridization in the primitive heart at 8.5 days postconception (p.c.) and in the myotome of the somites at 10.5 days p.c. This tissue-restricted pattern was corroborated by immunohistochemical studies. αB-crystallin mRNA and protein expression were uniform in the developing atria and ventricles without regional differences or gradients. αB-crystallin expression was absent in the endocardial cushion, pulmonary trunk, aorta, and endothelium. Examination of muscle precursors revealed expression throughout the dorsoventral aspect of the myotomes and in developing skeletal muscle. Our findings suggest that αB-crystallin may serve pivotal roles as a structural protein and a molecular chaperone in myofiber stabilization of metabolically active tissues during early embryogenesis. Thus, early αB-crystallin expression in myogenic lineages supports the hypothesis that the putative functions of αB- crystallin are coupled to the activation of genetic programs responsible for myogenic differentiation and cardiac morphogenesis.

AB - Although the small (22 Kd) heat shock protein/αB-crystallin functions as a major structural protein and molecular chaperone in the vertebrate lens, little is known about the protein's role in nonlenticular tissues such as the heart and skeletal muscle. Recent studies have demonstrated that αB- crystallin expression is uniquely regulated during myogenesis in vitro. We report here for the first time that the temporal and spatial expression of αB-crystallin is similarly regulated in vivo during mouse embryogenesis. Expression of αB-crystallin mRNA was detected by in situ hybridization in the primitive heart at 8.5 days postconception (p.c.) and in the myotome of the somites at 10.5 days p.c. This tissue-restricted pattern was corroborated by immunohistochemical studies. αB-crystallin mRNA and protein expression were uniform in the developing atria and ventricles without regional differences or gradients. αB-crystallin expression was absent in the endocardial cushion, pulmonary trunk, aorta, and endothelium. Examination of muscle precursors revealed expression throughout the dorsoventral aspect of the myotomes and in developing skeletal muscle. Our findings suggest that αB-crystallin may serve pivotal roles as a structural protein and a molecular chaperone in myofiber stabilization of metabolically active tissues during early embryogenesis. Thus, early αB-crystallin expression in myogenic lineages supports the hypothesis that the putative functions of αB- crystallin are coupled to the activation of genetic programs responsible for myogenic differentiation and cardiac morphogenesis.

KW - αB-crystallin

KW - development

KW - heart

KW - immunohistochemistry

KW - in situ hybridization

KW - mouse

KW - skeletal muscle

UR - http://www.scopus.com/inward/record.url?scp=0031031926&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031031926&partnerID=8YFLogxK

U2 - 10.1002/(SICI)1097-0177(199701)208:1<75::AID-AJA7>3.0.CO;2-Z

DO - 10.1002/(SICI)1097-0177(199701)208:1<75::AID-AJA7>3.0.CO;2-Z

M3 - Article

C2 - 8989522

AN - SCOPUS:0031031926

VL - 208

SP - 75

EP - 84

JO - Developmental Dynamics

JF - Developmental Dynamics

SN - 1058-8388

IS - 1

ER -