The γ isoform of CaM kinase II controls mouse egg activation by regulating cell cycle resumption

Johannes Backs, Paula Stein, Thea Backs, Francesca E. Duncan, Chad E. Grueter, John McAnally, Xiaoxia Qi, Richard M. Schultz, Eric N. Olson

Research output: Contribution to journalArticle

78 Citations (Scopus)

Abstract

Fertilization triggers a rise in intracellular Ca2+ concentration ([Ca2+]i) in the egg that initiates a series of events known as egg activation. These events include cortical granule exocytosis that establishes a block to polyspermy, resumption of meiosis, and recruitment of maternal mRNAs into polysomes for translation. Several calcium-dependent proteins, including calcium/calmodulin-dependent protein kinase II (CaMKII), have been implicated in egg activation. However, the precise role of CaMKII in mediating specific events of egg activation and the identity of the isoform(s) present in mouse eggs have not been unequivocally established. Through targeted deletion of the γ isoform of CaMKII, we find that CaMKIIγ is the predominant CaMKII isoform in mouse eggs and that it is essential for egg activation. Although CaMKIIγ-/- eggs exhibit a normal pattern of Ca2+ oscillations after insemination and undergo cortical granule exocytosis, they fail to resume meiosis or to recruit maternal mRNAs. Surprisingly, we find that the recruitment of maternal mRNAs does not directly depend on CaMKII, but requires elevated [Ca2+]i and metaphase II exit. We conclude that CaMKIIγ specifically controls mouse egg activation by regulating cell cycle resumption.

Original languageEnglish (US)
Pages (from-to)81-86
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume107
Issue number1
DOIs
StatePublished - 2010

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Calcium-Calmodulin-Dependent Protein Kinase Type 2
Ovum
Cell Cycle
Protein Isoforms
Stored Messenger RNA
Eggs
Exocytosis
Meiosis
Calcium-Calmodulin-Dependent Protein Kinases
Polyribosomes
Insemination
Metaphase
Fertilization
Calcium

Keywords

  • CaMKII
  • Fertilization
  • Metaphase II exit

ASJC Scopus subject areas

  • General

Cite this

The γ isoform of CaM kinase II controls mouse egg activation by regulating cell cycle resumption. / Backs, Johannes; Stein, Paula; Backs, Thea; Duncan, Francesca E.; Grueter, Chad E.; McAnally, John; Qi, Xiaoxia; Schultz, Richard M.; Olson, Eric N.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, No. 1, 2010, p. 81-86.

Research output: Contribution to journalArticle

Backs, Johannes ; Stein, Paula ; Backs, Thea ; Duncan, Francesca E. ; Grueter, Chad E. ; McAnally, John ; Qi, Xiaoxia ; Schultz, Richard M. ; Olson, Eric N. / The γ isoform of CaM kinase II controls mouse egg activation by regulating cell cycle resumption. In: Proceedings of the National Academy of Sciences of the United States of America. 2010 ; Vol. 107, No. 1. pp. 81-86.
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