The 21- and 23-kD forms of TCRζ are generated by specific ITAM phosphorylations

Nicolai S C Van Oers, Brett Tohlen, Bernard Malissen, Carolyn R. Moomaw, Steve Afendis, Clive A. Slaughter

Research output: Contribution to journalArticle

73 Citations (Scopus)

Abstract

The T cell receptor (TCR) ζ subunit contains three immunoreceptor tyrosine-based activation motifs (ITAMs) that translate effective extracellular ligand binding into intracellular signals by becoming phosphorylated into 21- and 23-kD forms. We report here that the 21-kD form of TCRζ is generated by phosphorylation of the tyrosines in the second and third ITAMs, whereas the 23-kD form is formed by the additional phosphorylation of the membrane-proximal ITAM tyrosines. The stable formation of the 21- and 23-kD species requires the binding of the tandem SH2 domains of ZAP-70. We also report that TCR-mediated signaling processes can proceed independently of either the 21- or 23-kD species of TCRζ.

Original languageEnglish (US)
Pages (from-to)322-328
Number of pages7
JournalNature Immunology
Volume1
Issue number4
StatePublished - Oct 2000

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Immunoreceptor Tyrosine-Based Activation Motif
T-Cell Antigen Receptor
Phosphorylation
Tyrosine
src Homology Domains
Ligands
Membranes

ASJC Scopus subject areas

  • Immunology

Cite this

Van Oers, N. S. C., Tohlen, B., Malissen, B., Moomaw, C. R., Afendis, S., & Slaughter, C. A. (2000). The 21- and 23-kD forms of TCRζ are generated by specific ITAM phosphorylations. Nature Immunology, 1(4), 322-328.

The 21- and 23-kD forms of TCRζ are generated by specific ITAM phosphorylations. / Van Oers, Nicolai S C; Tohlen, Brett; Malissen, Bernard; Moomaw, Carolyn R.; Afendis, Steve; Slaughter, Clive A.

In: Nature Immunology, Vol. 1, No. 4, 10.2000, p. 322-328.

Research output: Contribution to journalArticle

Van Oers, NSC, Tohlen, B, Malissen, B, Moomaw, CR, Afendis, S & Slaughter, CA 2000, 'The 21- and 23-kD forms of TCRζ are generated by specific ITAM phosphorylations', Nature Immunology, vol. 1, no. 4, pp. 322-328.
Van Oers NSC, Tohlen B, Malissen B, Moomaw CR, Afendis S, Slaughter CA. The 21- and 23-kD forms of TCRζ are generated by specific ITAM phosphorylations. Nature Immunology. 2000 Oct;1(4):322-328.
Van Oers, Nicolai S C ; Tohlen, Brett ; Malissen, Bernard ; Moomaw, Carolyn R. ; Afendis, Steve ; Slaughter, Clive A. / The 21- and 23-kD forms of TCRζ are generated by specific ITAM phosphorylations. In: Nature Immunology. 2000 ; Vol. 1, No. 4. pp. 322-328.
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