The adenovirus E1A N-terminal repression domain represses transcription from a chromatin template in vitro

Paul M. Loewenstein, Shwu Yuan Wu, Cheng Ming Chiang, Maurice Green

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4 Scopus citations

Abstract

The adenovirus repression domain of E1A 243R at the E1A N-terminus (E1A 1-80) transcriptionally represses genes involved in differentiation and cell cycle progression. E1A 1-80 represses transcription in vitro from naked DNA templates through its interaction with p300 and TFIID. E1A 1-80 can also interact with several chromatin remodeling factors and associates with chromatin in vivo. We show here that E1A 243R and E1A 1-80 can repress transcription from a reconstituted chromatin template in vitro. Temporal analysis reveals strong repression by E1A 1-80 when added at pre-activation, activation and early transcription stages. Interestingly, E1A 1-80 can greatly enhance transcription from chromatin templates, but not from naked DNA, when added at pre-initiation complex (PIC) formation and transcription-initiation stages. These data reveal a new dimension for E1A 1-80's interface with chromatin and may reflect its interaction with key players in PIC formation, p300 and TFIID, and/or possibly a role in chromatin remodeling.

Original languageEnglish (US)
Pages (from-to)70-75
Number of pages6
JournalVirology
Volume428
Issue number1
DOIs
StatePublished - Jun 20 2012

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Keywords

  • Ad E1A
  • Adenovirus
  • N-Terminal transcription repression domain
  • Reconstituted chromatin

ASJC Scopus subject areas

  • Virology

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