The Arf tumor suppressor gene promotes hyaloid vascular regression during mouse eye development

Robyn N. McKeller, Jennifer L. Fowler, Justine J. Cunningham, Nikita Warner, Richard J. Smeyne, Frederique Zindy, Stephen X. Skapek

Research output: Contribution to journalArticlepeer-review

101 Scopus citations

Abstract

A key tumor suppressor mechanism that is disrupted frequently in human cancer involves the ARF and p53 genes. In mouse fibroblasts, the Arf gene product responds to abnormal mitogenic signals to activate p53 and trigger either cell cycle arrest or apoptosis. Recent evidence indicates that Arf also has p53-Independent functions that may contribute to its tumor suppressor activity. Using Afl-/- and p53-/- mice, we have discovered a p53-independent requirement for Arf in the developmental regression of the hyaloid vascular system (HVS) in the mouse eye. Arf is expressed in the vitreous of the eye and is induced before HVS regression in the first postnatal week. In the absence of Arf, failed HVS regression causes a pathological process that resembles persistent hyperplastic primary vitreous, a developmental human eye disease thought to have a genetic basis. These findings demonstrate an essential and unexpected role for Arf during mouse eye development, provide insights into the potential genetic basis for persistent hyperplastic primary vitreous, and indicate that Arf regulates vascular regression in a p53-independent manner. The latter finding raises the possibility that Arf may function as a tumor suppressor at least in part by regulating tumor angiogenesis.

Original languageEnglish (US)
Pages (from-to)3848-3853
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume99
Issue number6
DOIs
StatePublished - Mar 19 2002

ASJC Scopus subject areas

  • General

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