The bacterial effector VopL organizes actin into filament-like structures

Jacob A. Zahm, Shae B. Padrick, Zhucheng Chen, Chi W. Pak, Ali A. Yunus, Lisa Henry, Diana R. Tomchick, Zhe Chen, Michael K. Rosen

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

VopL is an effector protein from Vibrio parahaemolyticus that nucleates actin filaments. VopL consists of a VopL C-terminal domain (VCD) and an array of three WASP homology 2 (WH2) motifs. Here, we report the crystal structure of the VCD dimer bound to actin. The VCD organizes three actin monomers in a spatial arrangement close to that found in the canonical actin filament. In this arrangement, WH2 motifs can be modeled into the binding site of each actin without steric clashes. The data suggest a mechanism of nucleation wherein VopL creates filament-like structures, organized by the VCD with monomers delivered by the WH2 array, that can template addition of new subunits. Similarities with Arp2/3 complex and formin proteins suggest that organization of monomers into filament-like structures is a general and central feature of actin nucleation. PaperFlick

Original languageEnglish (US)
Pages (from-to)423-434
Number of pages12
JournalCell
Volume155
Issue number2
DOIs
StatePublished - Oct 10 2013

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Actin Cytoskeleton
Actins
Monomers
Actin-Related Protein 2-3 Complex
Vibrio parahaemolyticus
Nucleation
Binding Sites
Dimers
Proteins
Crystal structure

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Zahm, J. A., Padrick, S. B., Chen, Z., Pak, C. W., Yunus, A. A., Henry, L., ... Rosen, M. K. (2013). The bacterial effector VopL organizes actin into filament-like structures. Cell, 155(2), 423-434. https://doi.org/10.1016/j.cell.2013.09.019

The bacterial effector VopL organizes actin into filament-like structures. / Zahm, Jacob A.; Padrick, Shae B.; Chen, Zhucheng; Pak, Chi W.; Yunus, Ali A.; Henry, Lisa; Tomchick, Diana R.; Chen, Zhe; Rosen, Michael K.

In: Cell, Vol. 155, No. 2, 10.10.2013, p. 423-434.

Research output: Contribution to journalArticle

Zahm, JA, Padrick, SB, Chen, Z, Pak, CW, Yunus, AA, Henry, L, Tomchick, DR, Chen, Z & Rosen, MK 2013, 'The bacterial effector VopL organizes actin into filament-like structures', Cell, vol. 155, no. 2, pp. 423-434. https://doi.org/10.1016/j.cell.2013.09.019
Zahm JA, Padrick SB, Chen Z, Pak CW, Yunus AA, Henry L et al. The bacterial effector VopL organizes actin into filament-like structures. Cell. 2013 Oct 10;155(2):423-434. https://doi.org/10.1016/j.cell.2013.09.019
Zahm, Jacob A. ; Padrick, Shae B. ; Chen, Zhucheng ; Pak, Chi W. ; Yunus, Ali A. ; Henry, Lisa ; Tomchick, Diana R. ; Chen, Zhe ; Rosen, Michael K. / The bacterial effector VopL organizes actin into filament-like structures. In: Cell. 2013 ; Vol. 155, No. 2. pp. 423-434.
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