The bipartite D. melanogaster twist promoter is reorganized in D. virilis

Duojia Pan, Scott A. Valentine, Albert J. Courey

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The pivotal role of twist in mesoderm determination in the Drosophila embryo depends upon two processes - the transcriptional activation of twist in the ventrally located mesodermal anlage and the regulation of downstream gene expression by the twist transcription factor. To elucidate the molecular mechanisms involved in these processes, we have compared both the coding and regulatory regions of the twist genes from Drosophila melanogaster and Drosophila virilis. Within the coding region, the basic-helix-loop-helix DNA binding and dimerization motif is highly conserved, consistent with the functional importance of this domain. A comparison of the transcriptional regulatory regions reveals a high degree of conservation in the more distal of the two ventral activator regions that have been mapped in the twist 5′ flanking region. On the other hand, the more proximal ventral activator region is absent at the corresponding position in the D. virilis twist gene. Instead, there is a region in the second intron of the D. virilis gene that resembles the proximal element of the D. melanogaster gene, in that it consists of little more than a series of whole and half binding sites for the dorsal morphogen. In transformation experiments, the intronic D. virilis element directs an expression pattern that is indistinguishable from that directed by the D. melanogaster proximal VAR. Thus, the twi genes from these two species appear to have evolved enhancer elements with very similar structural and functional properties. These findings suggest that apparently redundant spatially regulated enhancer elements may each play essential roles in fine tuning the level and/or pattern of gene expression.

Original languageEnglish (US)
Pages (from-to)41-53
Number of pages13
JournalMechanisms of Development
Volume46
Issue number1
DOIs
StatePublished - Jan 1 1994

Fingerprint

Drosophila melanogaster
Genes
Nucleic Acid Regulatory Sequences
Drosophila
Twist-Related Protein 1
5' Flanking Region
Gene Expression Regulation
Dimerization
Mesoderm
Introns
Transcriptional Activation
Embryonic Structures
Binding Sites
Gene Expression
DNA

Keywords

  • dorsal morphogen
  • Dorsal/ventral pattern formation
  • Drosophila embryogenesis
  • Promoter
  • Transcription
  • twist

ASJC Scopus subject areas

  • Developmental Biology
  • Developmental Neuroscience

Cite this

The bipartite D. melanogaster twist promoter is reorganized in D. virilis. / Pan, Duojia; Valentine, Scott A.; Courey, Albert J.

In: Mechanisms of Development, Vol. 46, No. 1, 01.01.1994, p. 41-53.

Research output: Contribution to journalArticle

Pan, Duojia ; Valentine, Scott A. ; Courey, Albert J. / The bipartite D. melanogaster twist promoter is reorganized in D. virilis. In: Mechanisms of Development. 1994 ; Vol. 46, No. 1. pp. 41-53.
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