The cardiac Na+-Ca2+ exchanger has two cytoplasmic ion permeation pathways

Scott A. John, Jun Liao, Youxing Jiang, Michela Ottolia

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The Na+-Ca2+ exchanger (NCX) is a ubiquitously expressed plasma membrane protein. It plays a fundamental role in Ca 2+ homeostasis by moving Ca2+ out of the cell using the electrochemical gradient of Na+ as the driving force. Recent structural studies of a homologous archaebacterial exchanger, NCX-Mj, revealed its outward configuration with two potential ion permeation pathways exposed to the extracellular environment. Based on the symmetry of NCX-Mj structure, an atomic model of an inward-facing conformation was generated showing similar pathways but directed to the cytoplasm. The presence of these water-filled cavities has yet to be confirmed experimentally, and it is unknown if the mammalian exchanger adopts the same structure. In this study, we mutated multiple residues within transmembrane segments 2 and 7 of NCX1.1 (cardiac isoform) to cysteines, allowing us to investigate their sensitivity to membrane-impermeable sulfhydryl reagents as exchanger current block. By trapping NCX1.1 in the inward-facing configuration, we have mapped two differently sized cytoplasmic aqueous cavities, the access of which is modified during exchange. This data reveals movements of the protein associated with ion transport. Electrophysiological characterization shows that the conserved residues within transmembrane segments 2 and 7, coordinating Na+ and Ca2+ ions in NCX-Mj, play a fundamental role in NCX1.1. Our results suggest a similar architecture between the mammalian and archaebacterial exchangers.

Original languageEnglish (US)
Pages (from-to)7500-7505
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume110
Issue number18
DOIs
StatePublished - Apr 30 2013

Fingerprint

Ions
Sulfhydryl Reagents
Ion Transport
Cysteine
Blood Proteins
Protein Isoforms
Membrane Proteins
Cytoplasm
Homeostasis
Cell Membrane
Membranes
Water
Proteins

Keywords

  • Ion coordination sites
  • Ion translocation pathways
  • MTS reagents
  • Sodium-calcium exchanger

ASJC Scopus subject areas

  • General

Cite this

The cardiac Na+-Ca2+ exchanger has two cytoplasmic ion permeation pathways. / John, Scott A.; Liao, Jun; Jiang, Youxing; Ottolia, Michela.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 110, No. 18, 30.04.2013, p. 7500-7505.

Research output: Contribution to journalArticle

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