The Ccr4-Not protein complex regulates the phase of the neurospora circadian clock by controlling WHITE COLLAR protein stability and activity

Guocun Huang, Qiyang He, Jinhu Guo, Joonseok Cha, Yi Liu

Research output: Contribution to journalArticle

3 Scopus citations


In the Neurospora circadian negative feedback loop, WHITE COLLAR 1 (WC-1) and WC-2 form the WC complex that activates frequency (frq) transcription. Here we show that Not1 is a WC-interacting protein and is important for maintaining WC levels. The not1 transcript displays a circadian oscillation with a similar phase as frq. Down-regulation of not1 leads to low levels of WC-1 and WC-2 and a delayed circadian phase as a result of increased protein degradation and increased WC activity. Protein purification of Not1 shows that it is part of the Neurospora Ccr4-Not complex. ccr4 is a clock-controlled gene and is regulated directly by the WC complex. Down-regulation of ccr4 results in a phase delay and period lengthening of the clock. Together, our findings suggest that the Ccr4-Not complex participates in the Neurospora clock function by interacting with and regulating the WC complex.

Original languageEnglish (US)
Pages (from-to)31002-31009
Number of pages8
JournalJournal of Biological Chemistry
Issue number43
StatePublished - Oct 25 2013


ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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