TY - JOUR
T1 - The cellular basis of adjuvant arthritis. I. Enhancement of cell-mediated passive transfer by concanavalin A and by immunosuppressive pretreatment of the recipient
AU - Taurog, Joel D.
AU - Sandberg, Gregory P.
AU - Mahowald, Maren L.
N1 - Funding Information:
’ This work was supported by Young Investigator Award AM29799-01 from the National Institutes of Health to Dr. Taurog; and by grants from the Minnesota Chapter, Arthritis Foundation; the Minnesota Medical Foundation; the Minnesota Chapter, Lupus Foundation of America; the Graduate School of the University of Minnesota; and the Medical School of the University of Minnesota. * To whom correspondence should be addressed.
PY - 1983/2/1
Y1 - 1983/2/1
N2 - Two reliable systems for the cell-mediated passive transfer of adjuvant arthritis have been developed. Donor rats were sensitized with Mycobacterium butyricum in mineral oil. In the first system, intravenous injection of adjuvant-sensitized donor lymph node or spleen cells into adultthymectomized, lethally irradiated, bone marrow cell-reconstituted syngeneic rats induced arthritis in the recipients. In the second system, adjuvant-sensitized donor lymph node or spleen cells were cultured in vitro with concanavalin A; these cells induced arthritis in normal recipients as well as in thymectomized, irradiated, bone marrow cell-reconstituted recipients. The passively transferred disease in both systems resembled classical adjuvant-induced arthritis clinically, radiographically, and histologically. Neither irradiated, adjuvant-sensitized donor cells nor cells from donors not injected with complete adjuvant could passively transfer arthritis.
AB - Two reliable systems for the cell-mediated passive transfer of adjuvant arthritis have been developed. Donor rats were sensitized with Mycobacterium butyricum in mineral oil. In the first system, intravenous injection of adjuvant-sensitized donor lymph node or spleen cells into adultthymectomized, lethally irradiated, bone marrow cell-reconstituted syngeneic rats induced arthritis in the recipients. In the second system, adjuvant-sensitized donor lymph node or spleen cells were cultured in vitro with concanavalin A; these cells induced arthritis in normal recipients as well as in thymectomized, irradiated, bone marrow cell-reconstituted recipients. The passively transferred disease in both systems resembled classical adjuvant-induced arthritis clinically, radiographically, and histologically. Neither irradiated, adjuvant-sensitized donor cells nor cells from donors not injected with complete adjuvant could passively transfer arthritis.
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U2 - 10.1016/0008-8749(83)90325-8
DO - 10.1016/0008-8749(83)90325-8
M3 - Article
C2 - 6600973
AN - SCOPUS:0020574933
SN - 0008-8749
VL - 75
SP - 271
EP - 282
JO - Cellular Immunology
JF - Cellular Immunology
IS - 2
ER -