The cellular homologue of the transforming gene of SKV avian retrovirus maps to human chromosome region 1q22→q24

R. S K Chaganti, I. Balazs, S. C. Jhanwar, V. V. Murty, P. R. Koduru, K. H. Grzeschik, E. Stavnezer

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

We report the chromosomal localization of the cellular oncogene SKI, the putative oncogene of the Sloan-Kettering viruses (SKVs), a group of transforming retroviruses that had been isolated from chicken embryo cells infected with the avian leukosis virus tdB77. Southern blot analysis of DNA from mouse x human somatic cell hybrids with the v-SKI probe established synteny with chromosome 1, but excluding the region 1pter→q21. In situ hybridization of the same probe both to human spermatocyte pachytene and lymphocyte metaphase chromosomes enabled precise localization of the gene to the region 1q22→q24, a region that frequently is involved in translocations and other rearrangements in diverse human tumor types. In situ hybridization studies of metaphase spreads from a small noncleaved cell lymphoma that exhibited a t(1;14)(q21;q32) translocation showed that SKI translocates to the der(14) chromosome. Cytogenetic analysis of 65 prospectively ascertained non-Hodgkin's lymphomas revealed that the SKI region undergoes nonrandom breakage leading to translocations. Further analysis of the chromosome breaks in this group of lymphomas suggested that those involving the SKI site probably are of importance in tumor progression.

Original languageEnglish (US)
Pages (from-to)181-186
Number of pages6
JournalCytogenetics and Cell Genetics
Volume43
Issue number3-4
StatePublished - 1986

Fingerprint

Human Chromosomes
Retroviridae
Oncogenes
Metaphase
Viruses
Non-Hodgkin's Lymphoma
In Situ Hybridization
Avian Leukosis Virus
Synteny
Chromosomes, Human, Pair 14
Chromosome Breakage
Spermatocytes
Hybrid Cells
Chromosomes, Human, Pair 1
Cytogenetic Analysis
Southern Blotting
Chickens
Lymphoma
Neoplasms
Embryonic Structures

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

Chaganti, R. S. K., Balazs, I., Jhanwar, S. C., Murty, V. V., Koduru, P. R., Grzeschik, K. H., & Stavnezer, E. (1986). The cellular homologue of the transforming gene of SKV avian retrovirus maps to human chromosome region 1q22→q24. Cytogenetics and Cell Genetics, 43(3-4), 181-186.

The cellular homologue of the transforming gene of SKV avian retrovirus maps to human chromosome region 1q22→q24. / Chaganti, R. S K; Balazs, I.; Jhanwar, S. C.; Murty, V. V.; Koduru, P. R.; Grzeschik, K. H.; Stavnezer, E.

In: Cytogenetics and Cell Genetics, Vol. 43, No. 3-4, 1986, p. 181-186.

Research output: Contribution to journalArticle

Chaganti, RSK, Balazs, I, Jhanwar, SC, Murty, VV, Koduru, PR, Grzeschik, KH & Stavnezer, E 1986, 'The cellular homologue of the transforming gene of SKV avian retrovirus maps to human chromosome region 1q22→q24', Cytogenetics and Cell Genetics, vol. 43, no. 3-4, pp. 181-186.
Chaganti, R. S K ; Balazs, I. ; Jhanwar, S. C. ; Murty, V. V. ; Koduru, P. R. ; Grzeschik, K. H. ; Stavnezer, E. / The cellular homologue of the transforming gene of SKV avian retrovirus maps to human chromosome region 1q22→q24. In: Cytogenetics and Cell Genetics. 1986 ; Vol. 43, No. 3-4. pp. 181-186.
@article{5cf022a7369442e6a4c16f5275c6c764,
title = "The cellular homologue of the transforming gene of SKV avian retrovirus maps to human chromosome region 1q22→q24",
abstract = "We report the chromosomal localization of the cellular oncogene SKI, the putative oncogene of the Sloan-Kettering viruses (SKVs), a group of transforming retroviruses that had been isolated from chicken embryo cells infected with the avian leukosis virus tdB77. Southern blot analysis of DNA from mouse x human somatic cell hybrids with the v-SKI probe established synteny with chromosome 1, but excluding the region 1pter→q21. In situ hybridization of the same probe both to human spermatocyte pachytene and lymphocyte metaphase chromosomes enabled precise localization of the gene to the region 1q22→q24, a region that frequently is involved in translocations and other rearrangements in diverse human tumor types. In situ hybridization studies of metaphase spreads from a small noncleaved cell lymphoma that exhibited a t(1;14)(q21;q32) translocation showed that SKI translocates to the der(14) chromosome. Cytogenetic analysis of 65 prospectively ascertained non-Hodgkin's lymphomas revealed that the SKI region undergoes nonrandom breakage leading to translocations. Further analysis of the chromosome breaks in this group of lymphomas suggested that those involving the SKI site probably are of importance in tumor progression.",
author = "Chaganti, {R. S K} and I. Balazs and Jhanwar, {S. C.} and Murty, {V. V.} and Koduru, {P. R.} and Grzeschik, {K. H.} and E. Stavnezer",
year = "1986",
language = "English (US)",
volume = "43",
pages = "181--186",
journal = "Cytogenetic and Genome Research",
issn = "1424-8581",
publisher = "S. Karger AG",
number = "3-4",

}

TY - JOUR

T1 - The cellular homologue of the transforming gene of SKV avian retrovirus maps to human chromosome region 1q22→q24

AU - Chaganti, R. S K

AU - Balazs, I.

AU - Jhanwar, S. C.

AU - Murty, V. V.

AU - Koduru, P. R.

AU - Grzeschik, K. H.

AU - Stavnezer, E.

PY - 1986

Y1 - 1986

N2 - We report the chromosomal localization of the cellular oncogene SKI, the putative oncogene of the Sloan-Kettering viruses (SKVs), a group of transforming retroviruses that had been isolated from chicken embryo cells infected with the avian leukosis virus tdB77. Southern blot analysis of DNA from mouse x human somatic cell hybrids with the v-SKI probe established synteny with chromosome 1, but excluding the region 1pter→q21. In situ hybridization of the same probe both to human spermatocyte pachytene and lymphocyte metaphase chromosomes enabled precise localization of the gene to the region 1q22→q24, a region that frequently is involved in translocations and other rearrangements in diverse human tumor types. In situ hybridization studies of metaphase spreads from a small noncleaved cell lymphoma that exhibited a t(1;14)(q21;q32) translocation showed that SKI translocates to the der(14) chromosome. Cytogenetic analysis of 65 prospectively ascertained non-Hodgkin's lymphomas revealed that the SKI region undergoes nonrandom breakage leading to translocations. Further analysis of the chromosome breaks in this group of lymphomas suggested that those involving the SKI site probably are of importance in tumor progression.

AB - We report the chromosomal localization of the cellular oncogene SKI, the putative oncogene of the Sloan-Kettering viruses (SKVs), a group of transforming retroviruses that had been isolated from chicken embryo cells infected with the avian leukosis virus tdB77. Southern blot analysis of DNA from mouse x human somatic cell hybrids with the v-SKI probe established synteny with chromosome 1, but excluding the region 1pter→q21. In situ hybridization of the same probe both to human spermatocyte pachytene and lymphocyte metaphase chromosomes enabled precise localization of the gene to the region 1q22→q24, a region that frequently is involved in translocations and other rearrangements in diverse human tumor types. In situ hybridization studies of metaphase spreads from a small noncleaved cell lymphoma that exhibited a t(1;14)(q21;q32) translocation showed that SKI translocates to the der(14) chromosome. Cytogenetic analysis of 65 prospectively ascertained non-Hodgkin's lymphomas revealed that the SKI region undergoes nonrandom breakage leading to translocations. Further analysis of the chromosome breaks in this group of lymphomas suggested that those involving the SKI site probably are of importance in tumor progression.

UR - http://www.scopus.com/inward/record.url?scp=0022912776&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022912776&partnerID=8YFLogxK

M3 - Article

C2 - 3026737

AN - SCOPUS:0022912776

VL - 43

SP - 181

EP - 186

JO - Cytogenetic and Genome Research

JF - Cytogenetic and Genome Research

SN - 1424-8581

IS - 3-4

ER -