The crystal structure of the bacterial chaperonin GroEL at 2.8 Å

Kerstin Braig, Zbyszek Otwinowskl, Rashmi Hegde, David C. Boisvert, Andrzej Joachimiak, Arthur L. Horwich, Paul B. Sigler

Research output: Contribution to journalArticle

1120 Citations (Scopus)

Abstract

The crystal structure of Escherichia coll GroEL shows a porous cylinder of 14 subunits made of two nearly 7-fold rotationally symmetrical rings stacked back-to-back with dyad symmetry. The subunits consist of three domains: a large equatorial domain that forms the foundation of the assembly at its waist and holds the rings together; a large loosely structured apical domain that forms the ends of the cylinder; and a small slender intermediate domain that connects the two, creating side windows. The three-dimensional structure places most of the mutationally defined functional sites on the channel walls and its outward invaginations, and at the ends of the cylinder.

Original languageEnglish (US)
Pages (from-to)578-586
Number of pages9
JournalNature
Volume371
Issue number6498
StatePublished - Oct 13 1994

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Chaperonins
Bacterial Structures
Escherichia

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Braig, K., Otwinowskl, Z., Hegde, R., Boisvert, D. C., Joachimiak, A., Horwich, A. L., & Sigler, P. B. (1994). The crystal structure of the bacterial chaperonin GroEL at 2.8 Å. Nature, 371(6498), 578-586.

The crystal structure of the bacterial chaperonin GroEL at 2.8 Å. / Braig, Kerstin; Otwinowskl, Zbyszek; Hegde, Rashmi; Boisvert, David C.; Joachimiak, Andrzej; Horwich, Arthur L.; Sigler, Paul B.

In: Nature, Vol. 371, No. 6498, 13.10.1994, p. 578-586.

Research output: Contribution to journalArticle

Braig, K, Otwinowskl, Z, Hegde, R, Boisvert, DC, Joachimiak, A, Horwich, AL & Sigler, PB 1994, 'The crystal structure of the bacterial chaperonin GroEL at 2.8 Å', Nature, vol. 371, no. 6498, pp. 578-586.
Braig K, Otwinowskl Z, Hegde R, Boisvert DC, Joachimiak A, Horwich AL et al. The crystal structure of the bacterial chaperonin GroEL at 2.8 Å. Nature. 1994 Oct 13;371(6498):578-586.
Braig, Kerstin ; Otwinowskl, Zbyszek ; Hegde, Rashmi ; Boisvert, David C. ; Joachimiak, Andrzej ; Horwich, Arthur L. ; Sigler, Paul B. / The crystal structure of the bacterial chaperonin GroEL at 2.8 Å. In: Nature. 1994 ; Vol. 371, No. 6498. pp. 578-586.
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