The developmentally restricted pattern of expression in the male germ line of a murine cyclin A, cyclin A2, suggests roles in both mitotic and meiotic cell cycles

Stuart E. Ravnik, Debra J. Wolgemuth

Research output: Contribution to journalArticle

73 Citations (Scopus)

Abstract

We have isolated cDNAs encoding a murine cyclin A, designated cyclin A2, and have examined its in vivo expression at the level of both mRNA and protein, with particular focus on the male germ line. Cyclin A2 is expressed in embryos and in a variety of adult tissues, including the testis. In the testis, however, a striking cellular specificity of expression was observed. At both the DNA and protein levels, the predominant sites of cyclin A2 expression were in the germ line stem cells, the spermatogonia, and in highest levels in preleptotene spermatocytes, cells in which premeiotic DNA synthesis occurs. The concurrent localization of cyclin A2 mRNA and protein further suggested that cyclin A2 is regulated at the level of transcription in these cells. The observed cellular specifcity of cyclin A2 expression is consistent with its function during mitosis in the stem cell stage of this lineage, while the restricted meiotic stage localization suggests function in G1/S or S but not in the meiotic divisions per se.

Original languageEnglish (US)
Pages (from-to)69-78
Number of pages10
JournalDevelopmental Biology
Volume173
Issue number1
DOIs
StatePublished - Jan 10 1996

Fingerprint

Cyclin A2
Cyclin A
Germ Cells
Cell Cycle
Testis
Stem Cells
Messenger RNA
Spermatogonia
Proteins
Spermatocytes
DNA
Cell Lineage
Mitosis
Embryonic Structures
Complementary DNA

ASJC Scopus subject areas

  • Developmental Biology

Cite this

@article{db03c9e9fc9c491b908f7cdc4c2549c1,
title = "The developmentally restricted pattern of expression in the male germ line of a murine cyclin A, cyclin A2, suggests roles in both mitotic and meiotic cell cycles",
abstract = "We have isolated cDNAs encoding a murine cyclin A, designated cyclin A2, and have examined its in vivo expression at the level of both mRNA and protein, with particular focus on the male germ line. Cyclin A2 is expressed in embryos and in a variety of adult tissues, including the testis. In the testis, however, a striking cellular specificity of expression was observed. At both the DNA and protein levels, the predominant sites of cyclin A2 expression were in the germ line stem cells, the spermatogonia, and in highest levels in preleptotene spermatocytes, cells in which premeiotic DNA synthesis occurs. The concurrent localization of cyclin A2 mRNA and protein further suggested that cyclin A2 is regulated at the level of transcription in these cells. The observed cellular specifcity of cyclin A2 expression is consistent with its function during mitosis in the stem cell stage of this lineage, while the restricted meiotic stage localization suggests function in G1/S or S but not in the meiotic divisions per se.",
author = "Ravnik, {Stuart E.} and Wolgemuth, {Debra J.}",
year = "1996",
month = "1",
day = "10",
doi = "10.1006/dbio.1996.0007",
language = "English (US)",
volume = "173",
pages = "69--78",
journal = "Developmental Biology",
issn = "0012-1606",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - The developmentally restricted pattern of expression in the male germ line of a murine cyclin A, cyclin A2, suggests roles in both mitotic and meiotic cell cycles

AU - Ravnik, Stuart E.

AU - Wolgemuth, Debra J.

PY - 1996/1/10

Y1 - 1996/1/10

N2 - We have isolated cDNAs encoding a murine cyclin A, designated cyclin A2, and have examined its in vivo expression at the level of both mRNA and protein, with particular focus on the male germ line. Cyclin A2 is expressed in embryos and in a variety of adult tissues, including the testis. In the testis, however, a striking cellular specificity of expression was observed. At both the DNA and protein levels, the predominant sites of cyclin A2 expression were in the germ line stem cells, the spermatogonia, and in highest levels in preleptotene spermatocytes, cells in which premeiotic DNA synthesis occurs. The concurrent localization of cyclin A2 mRNA and protein further suggested that cyclin A2 is regulated at the level of transcription in these cells. The observed cellular specifcity of cyclin A2 expression is consistent with its function during mitosis in the stem cell stage of this lineage, while the restricted meiotic stage localization suggests function in G1/S or S but not in the meiotic divisions per se.

AB - We have isolated cDNAs encoding a murine cyclin A, designated cyclin A2, and have examined its in vivo expression at the level of both mRNA and protein, with particular focus on the male germ line. Cyclin A2 is expressed in embryos and in a variety of adult tissues, including the testis. In the testis, however, a striking cellular specificity of expression was observed. At both the DNA and protein levels, the predominant sites of cyclin A2 expression were in the germ line stem cells, the spermatogonia, and in highest levels in preleptotene spermatocytes, cells in which premeiotic DNA synthesis occurs. The concurrent localization of cyclin A2 mRNA and protein further suggested that cyclin A2 is regulated at the level of transcription in these cells. The observed cellular specifcity of cyclin A2 expression is consistent with its function during mitosis in the stem cell stage of this lineage, while the restricted meiotic stage localization suggests function in G1/S or S but not in the meiotic divisions per se.

UR - http://www.scopus.com/inward/record.url?scp=0030020371&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030020371&partnerID=8YFLogxK

U2 - 10.1006/dbio.1996.0007

DO - 10.1006/dbio.1996.0007

M3 - Article

C2 - 8575639

AN - SCOPUS:0030020371

VL - 173

SP - 69

EP - 78

JO - Developmental Biology

JF - Developmental Biology

SN - 0012-1606

IS - 1

ER -