Fatty acids synthesized by rat epididymal fat-pad slices in the presence of [1-3H]glucose, [3-3H]glucose and tritiated water were degraded for the localization of tritium in the molecule. 75% of the tritium from [1-3H]glucose and 85% from [3-3H]glucose was present on the odd carbons. The incorporation of 15% of the tritium from [3-3H] glucose in the even positions was unexpected. Tritium from 3H2O was incorporated to the same extent in the odd and even positions of the carbon chain. The presence of tritium from water in the odd positions confirms the participation of DPNH hydrogen in reductive fatty acid synthesis in the intact adipose tissue cell. The results with 3H2O and [1-3H]glucose resemble those previously reported for liver and suggest that the mechanisms of fatty acid synthesis are essentially similar in the two tissues.
|Original language||English (US)|
|Number of pages||6|
|Journal||Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism|
|State||Published - Dec 7 1966|
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