The distribution of tritium in fatty acids synthesized from tritiated glucose and tritiated water by rat adipose tissue

Daniel W. Foster, Joseph Katz

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

Fatty acids synthesized by rat epididymal fat-pad slices in the presence of [1-3H]glucose, [3-3H]glucose and tritiated water were degraded for the localization of tritium in the molecule. 75% of the tritium from [1-3H]glucose and 85% from [3-3H]glucose was present on the odd carbons. The incorporation of 15% of the tritium from [3-3H] glucose in the even positions was unexpected. Tritium from 3H2O was incorporated to the same extent in the odd and even positions of the carbon chain. The presence of tritium from water in the odd positions confirms the participation of DPNH hydrogen in reductive fatty acid synthesis in the intact adipose tissue cell. The results with 3H2O and [1-3H]glucose resemble those previously reported for liver and suggest that the mechanisms of fatty acid synthesis are essentially similar in the two tissues.

Original languageEnglish (US)
Pages (from-to)422-427
Number of pages6
JournalBiochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism
Volume125
Issue number3
DOIs
StatePublished - Dec 7 1966

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Endocrinology

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