TY - JOUR
T1 - The effect of a wound care solution containing polyhexanide and betaine on bacterial counts
T2 - Results of an in vitro study
AU - Minnich, Kevin E.
AU - Stolarick, Rebecca
AU - Wilkins, Robert G.
AU - Chilson, Gary
AU - Pritt, Stacy L.
AU - Unverdorben, Martin
N1 - Funding Information:
Support for this research was provided by National Institutes of Health grants U01- DK57249 (to M.F.S.), N01-HV-48141 and R01-HV-62681 (both to J.L.W.), and HL45508 (to R.C.). We thank Dr. David Smith (Department of Anthropology, University of California, Davis) for generously providing Yavapai AI samples and useful discussions on this work. In addition, we thank Ripan Malhi (Department of Anthropology, University of California, Davis) for a critical reading of the manuscript.
PY - 2012/10
Y1 - 2012/10
N2 - Polyhexanide and betaine topical solution is used in the management of infected wounds as a cleaning agent. An in vitro study was conducted to examine the antimicrobial effects of a solution containing 0.1% of the antimicrobial agent polyhexanide and 0.1% of the surfactant betaine. Three batches of each product were tested, and culture results of 13 microorganisms were evaluated after 7, 14, and 28 days using USP <51> methodology. Growth reduction was identical at each day following exposure to the solution in all micro-organisms except Aspergillus brasiliensis. A range of 5.3-log to 5.8-log reduction was seen for the following micro-organisms: Staphylococcus epidermidis, Pseudomonas aeruginosa, Serratia marcescens, Candida albicans, S. aureus, vancomycin-resistant Enterococcus faecalis, Proteus mirabilis, Escherichia coli, methicillin-resistant S. aureus, Acinetobacter baumannii, Enterobacter cloacae, and E. faecalis. For A. brasiliensis, reductions were 2.1-log, 2.3-log and 2.8-log at 7, 14, and 28 days, respectively. The results of this study indicate a 4+ log inhibition of activity in 12 of 13 micro-organisms exposed to the solution. Research to elucidate the potential clinical effects of these observations is needed.
AB - Polyhexanide and betaine topical solution is used in the management of infected wounds as a cleaning agent. An in vitro study was conducted to examine the antimicrobial effects of a solution containing 0.1% of the antimicrobial agent polyhexanide and 0.1% of the surfactant betaine. Three batches of each product were tested, and culture results of 13 microorganisms were evaluated after 7, 14, and 28 days using USP <51> methodology. Growth reduction was identical at each day following exposure to the solution in all micro-organisms except Aspergillus brasiliensis. A range of 5.3-log to 5.8-log reduction was seen for the following micro-organisms: Staphylococcus epidermidis, Pseudomonas aeruginosa, Serratia marcescens, Candida albicans, S. aureus, vancomycin-resistant Enterococcus faecalis, Proteus mirabilis, Escherichia coli, methicillin-resistant S. aureus, Acinetobacter baumannii, Enterobacter cloacae, and E. faecalis. For A. brasiliensis, reductions were 2.1-log, 2.3-log and 2.8-log at 7, 14, and 28 days, respectively. The results of this study indicate a 4+ log inhibition of activity in 12 of 13 micro-organisms exposed to the solution. Research to elucidate the potential clinical effects of these observations is needed.
KW - Antimicrobial agent
KW - Betaine
KW - In vitro study
KW - Polyhexanide
KW - Wounds
UR - http://www.scopus.com/inward/record.url?scp=84867775058&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84867775058&partnerID=8YFLogxK
M3 - Article
C2 - 23037330
AN - SCOPUS:84867775058
SN - 0889-5899
VL - 58
SP - 32
EP - 36
JO - Ostomy Wound Management
JF - Ostomy Wound Management
IS - 10
ER -